中文摘要：

目的：建立一种简便、准确、改进的高效液相色谱（HPLC）方法，测定大鼠口服羟基红花黄色素A（HSYA）后粪便和尿液中HSYA的含量。方法：色谱条件：DikmaC18色谱柱（250mm×4．6mm，5μm）；流动相为：A泵，水相（0.5％三乙胺＋1％冰醋酸）；B泵，有机相（甲醇：乙腈=23：2）；梯度洗脱A：B为84：16（0．01min）→54．7：45．3（17．00min）→84：16（17．01min）→84：16（22．01min）；平衡时间5min；检测波长403nm；代谢笼收集大鼠给药后24h粪便和尿液。10％三氯乙酸沉淀蛋白及离心后取上清液进样。结果：粪便和尿液中HSYA分别在0．8μg·m^-1～200μg·mL^-1、2，0μg·mL^-1-500μg·mL^-1范围内线性关系良好；最低检测限分别为0．0016、0．0032μg·mL^-1；提取回收率为82．5％～90．2％；日内精密度小于3．4％，日间精密度小于4．8％。大鼠灌胃给予HSYA水溶液后，HSYA从粪便和尿液中24h累计排泄回收率分别为44．66％±8．0％、5．58％±1．3％。结论：本方法检测限低，分析简便、准确，适合于大鼠口服HSYA后的排泄研究。

英文摘要：

Objective： To develop a simple, rapid hydroxysafflor yellow A （HSYA） in feces and urine and accurate HPLC method for determination of of rats after oral administration of HSYA. Methods ： Biological samples were deproteinized with 10% perchloric acid. The supernatant after centrifuge was injected into a Shimadzu C：s （250×4.6 mm, i.d. 5μm） column. The mobile phase was a gradient system of 0. 5% （v/v） triethylamine and 1% （v/v） glacial acetic acid for pump A, and MeOH： Acetonitrile =23：2 （v/v） for pump B. The gradient system for analysis was A： B =84：16 （0.01 min）→54. 7：45.3 （ 17.00 min）→84：16 （ 17.01 min） →84：16 （22. O1 min）. Result： The linearity range was 0. 8 μg· mL^-1 - 200 μg·mL^-1 for feces and 2.0 μg·mL^-1 - 500 μg·mL^-1 for urine, separately; mean recovery was 82.5% -90. 2%; both of the inter-day and intra-day precisions were 〈 or = 4. 8%. After oral administration of HSYA aqueous solution, the cumulative amount of HSYA in 24 h in feces and urine accounted for （44. 66±8.0）% and （5.58 ± 1.3）%, respectively. Conclusion： This improved HPLC method was successfully applied to the excretion studies of hydroxysafflor yellow A after oral administration of HSYA.