为探索单色光影响肉鸡肌肉生长发育的机理,研究单色光对肉鸡肌肉生长、肌纤维发育和血清睾酮水平的影响。将260只0日龄AA肉鸡随机分为4组,分别在白(400-760nm)、红(660nm)、绿(560nm)、蓝光(480nm)下饲养49d,采用组织学技术和放射免疫法,检测肌纤维面积、密度和血清睾酮水平。结果如下:①质量:21日龄,绿光组胸肌、腿肌比其它组高6.46%~13.57%和6.37%~16.34%(P〈0.05);49日龄,蓝光组胸肌和蓝、绿光组腿肌比其它组高21.42%和16.67%~25.44%(P〈0.05)。②肌纤维面积:21日龄,绿光组胸肌和绿、红光组腿肌比其他组高23.19%~54.01%和33.46%~56.67%(P〈0.05);49日龄,蓝光组胸肌、腿肌比其他组高9.92%~21.69%和21.11%~57.44%(P〈0.05)。③肌纤维密度:21日龄,红光组胸肌和蓝光组腿肌比其他组高19.17%~42.94%和10.13%~49.33%(P〈0.05);49日龄,红、白光组胸肌和红光组腿肌比其他组高27.36%(P〈0.05)和57.02%~112.03%(P〈0.05)。④21日龄,蓝、绿光组的睾酮水平比红、白光组高35.37%~37.13%(P〈0.05);49日龄,蓝光组的睾酮水平比其他光组高14.36%~28.77%(P〈0.05)。研究表明单色光影响肉鸡生长,绿光和蓝光比红光和白光更能有效地促进肉鸡肌肉的生长、肌纤维的发育以及血清睾酮的分泌。
The effect of monochromatic light on muscle growth and muscle fiber development in broilers were studied, and the internal mechanism was discussed in this paper. A total of 260 newly hatched broilers were divided randomly into four light treatments, and were reared under blue (480 nm, BL), green (560 nm, GL), red (660 nm, RL) and white (400--760 nm, WL) light by using LED for 49 days, respectively. The developmental characters of muscle fiber and the concentration of testosterone were investigated by histological chemistry and radioimmunity. The results showed that. (1) At 21 days of age, weight of breast muscle and thigh muscle were significantly higher in GL group than that in other groups (6.46%-13.57% more in breast muscle and 6.37%--16.34% more in thigh muscle, P〈0.05). Until 49 days of age, weight of breast muscle and thigh muscle were significantly higher in BL and GL group than that in other groups (21.42% more in breast muscle and 16.67%--25.44% more in thigh muscle, P〈0.05). (2) At 21 days of age, the area of major pectoral muscle fiber in GL group and gastrocnemius muscle fiber in GL and RL group were higher than that in other groups (Increased by 23.19%--54.01% and 33.46%--56.67% resepectively, P〈0.05). Until 49 days of age, the area of major pectoral muscle and the gastrocnemius muscle fiber were higher in BL group than that in other groups (Increased by 9.92% 21. 69% and 21.11%--57.44% resepectively, P〈0.05). (3) At 21 days of age, the density of major pectoral muscle fiber in RL group and gastrocnemius muscle fiber in BL group were greater than that in others (Increased by19.17% 42.94% and 10.13% 49.33% resepectively, P〈0.05). Until 49 days of age, the density of major pectoral muscle fiber in WL and RL group and gastrocnemius muscle fiber in RL group were greater than that in others (Increased by 27.36% and 57.02%--112.03% resepectively,P〈0.05). (4) At 21 days of age, the testosterone concentration was 35.37% 37.13% greater in BL