中文摘要：

为了研究单色光对肉鸡视网膜和松果体黑素视蛋白基因（OPN4-1和OPN4-2）、视网膜绿敏感锥体视蛋白基因（CHK-1）和蓝敏感锥体视蛋白基因（CHK-2）mRNA转录的影响,笔者选用刚出壳的雄性AA肉鸡40羽,分别饲养在白光（400～700nm）、红光（660nm）、绿光（540nm）和蓝光（480nm）下2周,单色光源采用冷LED灯,光照度为15lx。于第14天分别取视网膜和松果体,提取总RNA,RT-PCR反转录后用上述4种肉鸡相关视蛋白基因的引物进行PCR扩增。结果显示：（1）视网膜和松果体蓝光组OPN4-1 mRNA的表达均显著较白光组高13.44%和16.77%（P〈0.05）,但视网膜中红光组和绿光组均分别显著较白光组低16.62%和10.94%（P〈0.05）,而松果体中绿光组与白光组差异不显著（P〉0.05）;（2）视网膜和松果体OPN4-2 mRNA表达均是红光组最高,其次是蓝光组,而绿光组均最低（P〈0.05）;（3）视网膜中4个光组CHK-1 mRNA表达均大于CHK-2mR-NA表达（P〈0.05）,其中绿光组CHK-1 mRNA表达分别显著较红光和蓝光组高24.37%和9.94%（P〈0.05）,但与白光组差异不显著;蓝光组CHK-2 mRNA表达分别显著较红光和绿光组高40.90%和8.94（P〈0.05）,但与白光组差异不显著（P〉0.05）。以上结果表明,不同视蛋白具有不同的光色敏感性。与红光和绿光相比,蓝光促进视网膜和松果体OPN4-1的转录。

英文摘要：

Aim of this study was to compare the effect of monochromatic light on transcription of melanopsin（OPN4-1 and OPN4-2）,green sensitive cone opsin（CHK-1） and blue sensitive cone opsin（CHK-2） genes in the retinas and pineal glands of broiler.Forty newly hatched male AA（Arbor Acres） broilers were exposed to white（400-760 nm）,red（660 nm）,green（560 nm） and blue light（480 nm） for 14 days by using LED（light-emitting diodes） with 15 lx light intensity as light sources,respectively.The total RNA of retinas and pineal glands was extracted respectively on the 14 th day.After reverse transcription,PCR amplification was carried out to detect transcription of above four opsin-related genes by using corresponding primers.The results showed：（1）OPN4-1 mRNA expression of BL group were 13.44% and 16.77% higher than that of WL group either in the retinas or pineal glands（P0.05） significantly,but OPN4-1 mRNA expression of RL and GL groups were 16.62% and 10.94% lower than that of WL group in the retinas（P0.05） significantly and no significant difference was detected between GL and WL groups in the pineal glands（P0.05）.（2）In the retinas and pineal glands,OPN4-2 mRNA expression of RL group was the highest,followed by BL group,and GL group was the lowest in all light groups（P0.05）.（3）In the retinas,the expression of CHK-1 mRNA was higher than that of CHK-2 under four monochromatic lights.CHK-1 mRNA expression of GL group was 24.37% and 9.94% higher than that of RL and BL groups（P0.05） and no significant difference was found between GL and WL groups（P0.05）.CHK-2 mRNA expression of BL group were 40.90% and 8.94% higher than that of RL and GL groups（P0.05） and no significant difference was found between BL and WL groups（P0.05）.Above results suggested that the different opsins have the different light color sensitivity.Compare to red and green light,blue light promote the transcription of OPN4-1 in the retinas and pineal glands.