目的建立脑络欣通胶囊的质量控制方法。方法采用TLC法对制剂中主要药材当归、红花进行定性鉴别;采用HPLC法对黄芪中的黄芪甲苷,三七中的三七皂苷R1、人参皂苷Rg1、人参皂苷Rh,进行定量测定。结果薄层色谱斑点清晰,在与对照品或对照药材相应的位置上显相同颜色的斑点,阴性对照无干扰;黄芪甲苷在4.0~20.0μg呈良好的线性关系,r=0.9992;三七皂苷R1、人参皂苷Rg1,、人参皂苷Rb,分别在0.4—2.0、0.08—8.0、0.08~8.0μg范围内线性关系良好,r分别为0.9930、0.9920、0.9987。结论所建立的方法简单准确、专属性强、重现性好,能够更有效地控制该制剂的质量。
AIM To establish quality control method of Naoluo Xintong Capsules. METHODS The chief components of the preparation, Angelicae sinensis Radix, Carthami Flos, were identified by TLC. The contents of astragaloside IV in Astragali Radix and notoginsenoside RI , Rodix et Rhizoma were determined by HPLC. RESULTS ginsenosides Rg1 and ginsenoside Rb1 in Notoginseng The spots in TLC were clear without interference. The linear range for astragaloside IV was 4.0 - 20. 0 μg ( r = 0. 999 2). The linear range for notoginsenoside R1 , gin- senosides Rg1 and ginsenoside Rb1 were 0.4 -2.0 μg, 0.08 -8.0 μg, 0.08 -8.0 μg, respectively. CONCLU- SION The method is simple, accurate, with high reproducibility, which can be used for the quality control of Na- oluo Xintong Capsules.