中文摘要：

以木炭为碳源，分别采用回流、微波及超声等不同方法制备碳量子点（Carbon quantum dots，CQDs）。比较不同方法的优劣并优化反应条件、考察不同因素对其荧光量子产率的影响。得到最佳制备方法，制得粒径较小的荧光CQDs，用钝化剂PEG2000修饰后，提高其荧光寿命和量子产率。将修饰后的CQDs应用于与牛血清白蛋白（Bovine serum albumin，BSA）的相互作用，采用紫外吸收光谱法和荧光光谱法探讨其相互作用机理。结果表明，经回流法所制CQDs的荧光量子产率最高，其与BSA之间的荧光猝灭为静态猝灭过程。

英文摘要：

Carbon quantum dots （CQDs） are a new fluorescent material with stable fluorescence, low toxicity and many other superior properties that make their applications in biology possible. CQDs in wood charcoal were created by a 5N HNO3 solution using three different methods, reflux, microwave and ultrasound methods. Factors that determine the fluorescence quantum yield were examined and preparation conditions were optimized. Results indicated that the fluorescence quantum yield of CQDs prepared by the reflux method was highest among the three methods. The CQDs prepared by this method were modified by poly（ ethylene glycol） 2000 to increase the fluorescent lifetime and the quantum yield. The reaction of the modified CQDs with bovine serum albumin was investigated by ultraviolet absorption spectrometry and fluorescence spectrometry, which revealed a static quenching behavior.