中文摘要：

研究CdTe量子点（供体）和罗丹明B（受体）之间荧光共振能量转移的最佳条件,建立了荧光共振能量转移猝灭法测定金银花中微量铜的新方法。采用十六烷基三甲基溴化铵,在pH 6.00的Tris-HCl缓冲液中,Cu2＋能对能量转移体系中罗丹明B荧光峰强猝灭从而测定铜的含量。Cu2＋浓度在1.3×10-4～3.1×10-2μg.mL-1范围内与罗丹明B的荧光强度变化ΔF呈现良好线性关系（r=0.996）,检测限为4.6×10-5μg.mL-1,RSD为3.5%,平均回收率为103.4%（n=5）。该方法适用于金银花中微量铜的测定。

英文摘要：

The present work is to find the optimum condition for fluorescence resonance energy transfer between CdTe QDs（donor） and rhodamine B（acceptor） and form a new method to detect copper in Flos Lonicerae Japonicae.In tris-HCl buffer solution at pH 6.00,by employing cetyltrimethylammonium bromide,Cu2＋ can quench the fluorescence intensity of BRB and detect the concentration of Cu2＋.Results show that a linear relationship could be established between the quenched fluorescence intensity of RhB and the concentration of Cu2＋ in the range of 1.3×10-4~3.1×10-2 μg·mL-1,the limit of detection was 4.6×10-5 μg·mL-1（r=0.996）,RSD and the average recovery was 3.5% and 103.4%（n=5）,respectively.It is concluded that the developed method was proved to be feasible to detect copper in Flos Lonicerae Japonicae.