本研究基于中国地区双翅目寄蝇科寄蝇亚科5族10属17种昆虫的28SrRNA基因序列,分别利用PAUP4.0b和MEGA5.05软件的邻接法(NJ)、最大简约法(MP)和最小进化法(ME),选取追寄蝇亚科的黄足突额寄蝇Biomeigenia flava和麻蝇科的红尾拉麻蝇Ravinia sfriata为外群,重建寄蝇亚科部分种类的系统发育树。结果表明:测得的696bp寄蝇亚科核苷酸片段,包括127个变异位点和52个简约信息位点;不同方法得到的系统发育树的拓扑结构基本一致。本研究结果部分解决了基于形态学定义的该亚科内族问、属问的系统发育关系:(1)支持寄蝇亚科、寄蝇族分别为一个单系群;(2)支持形态分类的将诺寄蝇属Nowickia Wachtl并入寄蝇属Tachina Meigen并作为后者的一个亚属的结论;(3)同时支持将短须寄蝇族Linnaemyini并入埃内寄蝇族Ernestiini。
In this study, we used Neighbor-joining (N J), Maximum-parsimony (MP) and Minimum-evolution (ME) methods of PAUP4.0b and MEGAS.05 softwares, respectively selecting Biome ige nia flay a in subfamily Exoristinae and Ravinia striata in family Sarcophagidae as out-groups, re-establishing different kinds ofphylogenetic trees for Tachininae. This research was based on 28S rRNA gene sequences of 17 species (Diptera: Tachinidae: Tachininae) which were in 10 genera, 5 tribes in China. The results showed that the 696 bp nucleotide fragments of the subfamily included 127 mutation sites and 52 simple information sites, and the topological structures of phylog- enetic trees which were gotten by adopting various methods were basically coincide. The results partially solved the relationships of main tribes and genera of Tachininae defined by morphology, proved that Tachininae and Tachinini could respectively belong to one monophyletic group, supported that Nowickia Wachtl should be fallen under Tachina Meigen as its subgenus, and also provided evidence on a molecular level for combining Linnae- myini and Ernestiini into one tribe.