中文摘要：

对甘蔗（Saccharumofficinarum L．）叶片全长cDNA文库进行测序，获得了1个细胞质型苹果酸脱氢酶（cMDH）基因的全长cDNA序列，命名为Sc—cMDH。生物信息学分析表明，该基因全长1314bp，开放阅读框为999bp，编码332个氨基酸。Sc—cMDH与其他植物cMDH的氨基酸序列同源性高达86．5％～97．0％。Sc．cMDH包含典型的NAD结合基元T“GAAGQI”和催化基元1184WGNH’船，还有相当保守的6个半胱氨酸残基，因此推断该基因为细胞质型NAD—MDH。定量PCR分析结果表明，该基因在甘蔗叶片和根中的表达量高于茎。

英文摘要：

A full-length cDNA sequence of sugarcane （Saccharum officinarum L.） cytosolic malate dehydrogenase （cMDH） gene, named as Sc-cMDH, was obtained from sugarcane full-length cDNA library by EST sequencing and bioinformatics analysis. The full-length of Sc-cMDH was 1314 bp with 999 bp open reading frame, encoding 332 amino acids. Sc-cMDH shared 86.5%-97.0% of amino acids with cMDH from other 9 species. Sc-cMDH contains typical NAD＋ binding motif （THGAAGQ117） and catalytic motif （II84WGNH188）. Real-time PCR analysis showed that the expression of Sc-cMDH was higher in leaves and roots than that in stems of sugarcane.