选用燕麦、大豆、玉米、麸皮、大米、荞麦6种培养基质对灰树花孔菌进行固体发酵及抗氧化活性研究。结果表明:灰树花孔菌最佳抗氧化发酵基质为燕麦大豆培养基(燕麦16.25%,大豆83.75%),最佳发酵时间为8d。灰树花孔菌燕麦大豆发酵基质抗氧化活性成分种类丰富,含量高。其中总黄酮物质40.86μg/g,总三萜5.94mg/g,多酚8.51mg/g,还原糖13.10mg/g,花色苷70.23μg/g,维生素C24.57mg/g,维生素E0.33mg/g,谷光甘肽(GSH)0.84g/g,SOD酶活性299.74U/g。
Grifola frondosa was cultured on the solid medium using oat,wheat bran,soybeans,corn,rice and buckwheat as substrates.The best fermentation time was determined and antioxidant components of substrate was analyzed.The results show that the best antioxidant fermentation substrate of Grifola frondosa was oats and soybean(16.25% oats and 83.75% soybean),and the best fermentation time was 8d.The antioxidant components of Grifola frondosa in oat and soybean medium were rich in varieties and high in contents,which included flavonoids 40.86μg/g,total triterpenoid 5.94mg/g,total polyphenols 8.51mg/g,reducing sugar 13.10mg/g,anthocyanins 70.23μg/g,Vitamin C 24.57mg/g,Vitamin E 0.33mg/g.and glutathione(GSH) 0.84g/g,and SOD activity was 299.74U/g.