中文摘要：

研究了波茨坦短芽孢杆菌降解间甲酚的特性及双底物体系中苯酚和间甲酚的相互作用。结果表明,波茨坦短芽孢杆菌能在72 h内降解400 mg/L的间甲酚,但其降解间甲酚的能力低于苯酚,拟合的Haldane方程各动力学参数为：μ_（max）=0.226 h~（-1）,K_s=18.19 mg/L,K_i=130.12 mg/L。在苯酚-间甲酚体系中,苯酚抑制间甲酚的降解,同样间甲酚也抑制苯酚的降解;但是低浓度苯酚能促进间甲酚的降解,苯酚浓度150~300 mg/L时,能促进200 mg/L间甲酚的降解,苯酚200 mg/L时,间甲酚降解速率最快;动力学参数表明间甲酚对苯酚降解的抑制要强于苯酚对间甲酚降解的抑制。酶活测定表明,苯酚和间甲酚降解都遵循邻位降解途径：生成（取代的）邻苯二酚,然后在邻苯二酚1,2-双加氧酶作用下邻位开环裂解。

英文摘要：

Biodegradation characteristics of m-cresol by Brevibacillus borstelensis and interaction between phenol and mcresol in the dual-substrate biodegradation system were investigated. The results showed that Brevibacillus borstelensis could degrade 400 mg/L m-cresol in 72 h. The capacity to degrade m-cresol was obviously lower than that to degrade phenol. The parameter values of Haldane kinetic models were： μ_（max）=0.226 h~（-1）, K_s=18.19 mg/L, K_i=130.12 mg/L. When cells grew on the mixture of phenol and m-cresol, phenol inhibited the utilization of m-cresol; on the other hand, m-cresol also inhibited the degradation of phenol. However, the presence of low-concentration phenol enhanced m-cresol biodegradation; 200 mg/L mcresol could be completely degraded within a shorter period of time than m-cresol alone in the presence of 150 ~300 mg/L phenol. The maximum m-cresol biodegradation rate was obtained at the existence of 200 mg/L phenol. Furthermore, the obtained kinetic parameter values indicated that m-cresol inhibited phenol utilization more than phenol inhibited m-cresol utilization. In addition, enzyme activities measurement confirmed that phenol and m-cresol degradation by the strain proceeded through a same pathway involving the production of intermediates such as catechol or substituted catechol, which was degraded then via the pathway of ortho-cleavage catalyzed by catechol 1,2-dioxygenase.