中文摘要：

目的探讨P-选择素基因（P-selectin）缺失对小鼠生理特征的影响。方法 P-选择素敲除（PKO）小鼠和C57小鼠饲养于SPF级环境中,通过基因鉴定确定其为纯和PKO小鼠。观察PKO小鼠的活动状态、繁育状况,检测血常规,确定P-选择素敲除后对小鼠一般情况的影响。分别于6周和18周处死小鼠,通过伊红＆苏木素（HE）染色,观察P-选择素敲除后对小鼠各个脏器组织结构的影响。结果 PKO小鼠与正常C57小鼠的繁育状况相似,血常规无异常,HE染色发现PKO小鼠肝、脾、肺、肾组织结构均较正常。结论 P-选择素的缺失不会对小鼠生殖繁育、血液造成影响,对重要的组织脏器也未见明显影响,为以后进一步研究P-选择素在疾病中的作用提供了动物模型研究理论基础。

英文摘要：

Objective To investigate the effects of P-selectin gene knockout on the biological characteristics of mice. Methods P-selectin knockout（ PKO） mice and C57 BL /6 mice were housed in SPF environment. To make sure that the mice were pure PKO mice by gene identification. To determine the general condition,the activity status,reproduction of P-selectin knockout mice were observed and tested. HE staining was used to observed the histological morphology and structures of 6-week-old and 18-week-old P-selectin knockoutout mice. Results PKO mice and C57 mice have similar multiplication,there was no significant difference in reproduction and blood routine test. The stuctures of liver,spleen,lung and kidney were normal. Conclusions P-selectin knockout has no significant effects on reproduction,blood routine test and major organs. This research provides animal model theoretical basis for further study on the effects of P-selection in diseases.