中文摘要：

目的：通过测定卵巢癌细胞系Skov3多细胞团簇对细胞外基质（ECM）和人腹膜间皮细胞的黏附性，以及β1整合素抗体对此黏附性的影响，探讨多细胞团簇在卵巢癌扩散和转移中的作用，以及β1整合素的作用。方法：Skov3团簇通过液体重叠培养系统获得。无血清的Skov3团簇悬液加入覆以ECM、牛血清蛋白（BSA，作为对照）或单层腹膜间皮细胞的96孔板，镜下记数团簇，培养6或12h。PBS洗摔未黏附细胞，同法记数。为了检测β1-整合素对团簇黏附的影响，团簇悬液中分别加入CD29mAb或正常鼠免疫球蛋白（mIgG，作为对照）培养30min，再同法培养、记数。结果：Skov3团簇对ECM的黏附率为38％±12％，而对BSA的黏附率为8％±16％，P=0．001。mIgG组黏附率为37％±10％，CD29mAb组黏附率为14％±3％，P一0．001，CD29mAb的黏附抑制率为60％。团簇黏附于单层间皮细胞，mIgG组黏附率为41％±11％，而CD29mAb组黏附率为17％±5％，P=0．001。CD29mAb的抑制率为55％±2％。结论：本实验结果提示：卵巢癌多细胞团簇可通过β1整合素黏附于ECM和人腹膜间皮细胞。因此，团簇在卵巢癌播散和转移中具有不可忽视的作用。β1整合素介导这种黏附，阻断β1整合素的功能，可部分阻断团簇的黏附。提示，抗细胞黏附可能为治疗卵巢癌提供一种新方法。

英文摘要：

OBJECTIVE： To evaluate the role of Skov3 spheroids in spreading and metastasis of ovarian cancer, the ability of adhesion rate of Skov3 spheroids to extracellular matrix （ECM） and the ability of adhesion to live human mesothelial cells, and the role of β1 integrin subunit （CD29） in mediating the adhesion. METHODS： The spheroids were generated from the human ovarian carcinoma cell line Skov3 by using a liquid overlay technique. Adhesion assays of spheroids to ECM and monolayer of human mesothelial cell were performed as follows.. Spheroids in serurrn-free media were incubated on 96-wells coated with ECM or hove serum albumin （BSA） for 6h or with monolayer of human mesothelial cell for 12 h. Non-adherent spheroids were rinsed away with 1 × PBS, and the remaining adherent spheroids were counted. In order to identify the contribution of β1 integrin in mediating spheroids adhering, the assays were performed rate in the presence of the monoclonal antibody against the β1 integrin （CD29 mAb） or mIgG. RESULTS： The adhesion of Skov3 spheroids to ECM was 38% ± 12%, which was much higher than that of the BSA control group （8% ± 16%, P=0. 001）. The adhesion rate in the presence of mIgG was 37% ± 10%, however, the adhesion rate in the presence of CD29 mAb was only 14 % ± 3 %, P=0. 001. The adhesion of the spheroids to ECM was inhibited by 60% in the presence of CD29 mAb. Additionally, the adhesion rate of spheroids to monolayer of human mesothelial cell in present of mIgG was 41%±11%,while the adhesion rate in present of CD29 mAb was 17% ± 5%, P=0. 001. So the β1 integrins inhibited the adhesion by 55% ±2%. CONCLUSION.. Our findings suggest that avarian cancer muhicell spheroids can adhere to ECM and human mesothelial cells via β1 integrins, indicating that spheroids should not be ignored in the dissemination of ovarian cancer, and also suggesting a major role of β1 integrin in mediating the adhering process. It implies that anti-adhesion cancer therapy may be a new strategy for ovarian