中文摘要：

瞄准：在 hepatocellular 癌(HCC ) 的 carcinogenesis 调查 alpha fetoprotein (法新社) 和它的临床的意义的生物角色。方法：HCC 病人和 immunohistochemical 检查的临床的分析被进行评估在浆液法新社水平和耐心的死亡之间的关系。共焦的显微镜学，西方的弄污， dimethylthiahzolyl-2,5-diphenyl-tetrazolium 溴化物，房间数 Kit-8 试金和流动 cytometry 被执行探索可能的机制。结果：在在这研究注册的 160 个 HCC 病人之中， 130 个病人熬过 2 年(81.25%)，与在法新社的86.8%的幸存率< 2 0 g/L 组，88.9%在法新社 20-250 g/L 组织，并且69.6%在法新社> 250 g/L 组，与更高的法新社层次在 HCC 病人表明更高的死亡率。外科的治疗在有低法新社层次的病人仅仅是有益的。通过手术被对待的有高法新社层次的 HCC 病人的死亡率比与保守管理对待的那些显然高。immunohistochemistry 的结果证明那法新社和法新社受体仅仅与积极浆液法新社在 HCC 病人的纸巾被表示。一致地，在 vitro，分析显示出那个法新社， AFPS 在 HepG2 然而并非在 HLE 房间被表示。法新社显示了一个能力支持房间生长，并且这在 HepG2 房间，增长被 3.5 褶层在增加是更明显的。房间周期分析证明在在到法新社的暴露以后的 S 阶段的 HepG2 房间的百分比平常地被增加。结论：有更高的法新社层次的 HCC 病人显示出更高的死亡率，它看起来对支持法新社的性质的生长可归因。

英文摘要：

AIM：To investigate the biological role of alpha fetoprotein （AFP） and its clinical signif icance in carcinogenesis of hepatocellular carcinoma （HCC）.METHODS：Clinical analysis of HCC patients and im-munohistochemical examination were conducted to evaluate the relationship between serum AFP level and patient mortality. Confocal microscopy,Western blotting, dimethylthiahzolyl-2,5-diphenyl-tetrazolium bromide,Cell Counting Kit-8 assays and flow cytometry were performed to explore the possible mechanism.RESULTS： Among the 160 HCC patients enrolled in this study,130 patients survived 2 years （81.25%）,with a survival rate of 86.8% in AFP 〈 2 0 μg/L group,88.9% in AFP 20-250 μg/L group,and 69.6% in AFP 〉 250 μg/L group, demonstrating a higher mortality rate in HCC patients with higher AFP levels. Surgical treatment was benef icial only in patients with low AFP levels.The mortality rate of HCC patients with high AFP levels who were treated surgically was apparently higher than those treated with conservative management.The results of immunohistochemistry showed that AFP and AFP receptor were merely expressed in tissues of HCC patients with positive serum AFP.Consistently,in vitro analysis showed that AFP and AFPS were expressed in HepG2 but not in HLE cells. AFP showed a capability to promote cell growth,and this was more apparent in HepG2 cells,in which the proliferation was increased by 3.5 folds. Cell cycle analysis showed that the percent-age of HepG2 cells in S phase after exposure to AFP was modestly increased.CONCLUSION：HCC patients with higher AFP levels show a higher mortality rate,which appears to be attributable to the growth promoting properties of AFP.