中文摘要：

目的利用纳米金独特的生物和光学特性，合成一种新型纳米金荧光双标survivin反义寡核苷酸探针，观察其对人恶性黑素瘤A375细胞的影响。方法用柠檬酸钠还原法制备纳米金，将巯基修饰后的survivin反义寡核苷酸结合在纳米金表面，合成纳米金survivin反义寡核苷酸探针；再结合互补的带荧光标记的报告序列，制成纳米金荧光双标survivin反义寡核苷酸探针；将制备的探针转染人恶性黑素瘤A375细胞，荧光显微镜观察细胞内荧光图像，MTT法及流式细胞仪检测A375细胞的增殖及凋亡情况。结果制备的纳米金荧光双标survivin反义寡核苷酸探针转染A375细胞后，可观察到清晰的细胞内荧光图像，可以抑制A375细胞的增殖，与对照组比较差异有统计学意义（P〈0．05），诱导细胞凋亡增多。结论寡核苷酸功能化的纳米金颗粒可有效转染进人恶性黑素瘤A375细胞，通过反义封闭作用诱导人恶性黑素瘤A375细胞凋亡增多。

英文摘要：

Objective To synthesize the probe of survivin antisense oligonucleotide modified gold nanoparticles by making use of the biological and optical characteristics of gold nanoparticles and to analyze its impact on A375 cells. Methods Citrate-stabilized gold nanoparticles were prepared using published procedures. Thiol-modified oligonucleotides were added to gold colloids to make nano-probes. Then the complementary reporter sequence was combined to prepare ＂nano-flare＂ probes. A375 cells were grown with media containing nano-flares probes. Fluorescent images were observed under the fluorescence microscope. Cell proliferation was measured by MTT and cell apoptosis was detected by Annexin V/PI double staining method. Results The nano-probes could inhibit A375 cell proliferation significantly （P~0.05） and induce cell apoptosis. Conclusion The nano-probes that can be stably synthesized can inhibit proliferation of A375 cells and induce their apoptosis.