中文摘要：

目的研究西安地区2009年捕获鼠类种类和携带汉坦病毒情况,分析汉坦病毒的基因特征。方法在西安地区近年来肾综合征出血热高发的户县、长安两区县以夹夜法捕鼠,记录鼠种和生存环境。无菌解剖后取鼠肺提取总RNA,扩增汉坦病毒M片段基因进行病毒分型,选取部分阳性标本进行病毒S片段和L片段序列测定和进化分析。结果 2009年两区县捕获鼠类共400只,其中黑线姬鼠244只,占61%,褐家鼠89只,占22.25%,小家鼠43只,占10.75%,其他鼠种24只,占6%;共检出汉坦病毒核酸阳性鼠肺86只,分型以汉滩型为主,占79.07%;病毒L片段与贵州代表株同源性为95%97%,S片段与贵州代表株同源性为97.7%99.3%。结论 2009年西安地区汉坦病毒宿主以黑线姬鼠、褐家鼠、小家鼠为主,汉坦病毒核酸阳性率较高;所携带病毒以汉滩型为主,病毒进化分析与贵州代表株高度同源。

英文摘要：

Objective To study the captured murids in Xi’an in the year 2009 and the Hantavirus serotypes and genetic characteristics carried by these rodents.Methods Night trapping method was used to capture rats in Chang’an county and Hu county.Then we recorded the murids and their living environments,extracted RNA and genotyping of Hantavirus carried by the rodents,and sequenced the partial L and S segments to make the sequence alignment.Results Altogether 400 rats were captured in these two counties in 2009;Apodmus agrarius（61%）,Rattus norvegicus（22.25%） and Mus musculus（10.75%） were the main murids.21.5% of these rodents carried Hantavirus nucleotide and the results of virus genotyping was Hantaan virus.The sequence alignment results of the partial L and S segments showed that these sequences had the highest homology with S2/L4 Hantaan viruses group found in Guizhou Province,which were 95.0%-97.0% and 97.7%-99.3%,respectively.Conclusion The hosts of Hantavirus in Xi’an in 2009 were mainly Mus musculus,Apodmus agrarius,Rattus norvegicus,and the main genotype these rodents carried was Hantaanvirus,and the hosts had a relatively high virus-carrying rate.The virus strains were closely related to Hantaanviruses found in Guizhou.