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我国海域耳鲍(Haliotis asinisna)精子发生的超微结构研究
  • ISSN号:0029-814X
  • 期刊名称:《海洋与湖沼》
  • 时间:0
  • 分类:Q954.43[生物学—动物学]
  • 作者机构:[1]海南大学海洋学院,海口570228, [2]湖南城市学院化学与环境工程系,益阳413000, [3]海南大学实验中心,海口570228
  • 相关基金:国家“863”资源环境技术领域项目资助,2004AA603130号;国家自然科学基金项目资助,40366001号;教育部科学技术研究重点项目资助,03146号;海南省外国专家局资助项目,20031211号.
中文摘要:

应用透射电子显微镜技术研究了我国海域耳鲍精子发生的全过程.结果表明,耳鲍精原细胞呈近椭圆形,染色质分布较均匀,线粒体较少;初级精母细胞较大,染色质凝聚成小块状,线粒体增多;次级精母细胞较初级精母细胞小,线粒体较多,部分线粒体发生融合,呈扁囊状.分化早期精细胞染色质凝聚成团块状,多数贴附于核膜内面,胞质中出现前顶体颗粒.分化中期精细胞染色质继续凝聚成较大的团块,线粒体在核的一端融合,形成数量少、体积大的线粒体,前顶体颗粒形成圆形外膜明显的前顶体.分化后期精核由近圆形变成长桶状,核内染色质凝聚并均质化,前顶体泡化发育成顶体,鞭毛形成,精细胞分化成精子.成熟精子为鞭毛型精子,由头部、中段和尾部(鞭毛)3部分组成.

英文摘要:

For promoting and improving maricuhure of Haliotis asinine Linnaeus, an economically important nutrient abalone that grows fast in high yield, spermatogenesis of the abalone collected in the South China Sea near Hainan was studied using transmission electron microscopy (TEM). The results showed that the spermatogonium was oval with few mitochondria. The chromatin was relatively dispersed. The primary spermatocyte was larger than the spermatogonium ; its chromatin became denser and lied at one side of the nucleus ; the number of mitochondria increased. The secondary spermatocyte was usually smaller than the primary spermatocyte, many mitochondria merged into flat capsules. The differentiation of spermatid was complicated and could be divided into 3 stages in terms of acrosome evolvement. In the early stage, chromatin agglutinated into lumps and most of them were attached to the internal surface of nuclear membrane;the proacrosomic granule appeared in the cytoplasm ;the centriole moved to the posterior extremity of nucleus, and mitochondrion located at one side of the nucleus. In the middle stage, the chromatin continued to agglutinate into bigger lumps, the mitochondria fused at one side of nucleus and formed a few big mitoehondria complexes whose pro-acro- somic granule developed into a pre-acrosome that was round and surrounded by plasmalemma in homogeneously high electron density. In the late stage, the nucleus changed in shape from round to long ellipse with condensed and homogenized chromatin, the pre-acrosome approached to the nucleus, alveolated, and developed into acrosome. 5 cystiform mitochondria surrounded the centriole, flagellum formed, and at last the spermatid altered into spermatozoon. A mature spermatozoon was typical hauch-sperm, including a head, middle sector and tail (flagellum). The axoneme of flagellum featured in 9 + 2 microtubular pattern.

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期刊信息
  • 《海洋与湖沼》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学院
  • 主办单位:中国海洋湖沼学会
  • 主编:孙松
  • 地址:青岛市南海路7号中科院海洋所内
  • 邮编:266071
  • 邮箱:
  • 电话:0532-82898753
  • 国际标准刊号:ISSN:0029-814X
  • 国内统一刊号:ISSN:37-1149/P
  • 邮发代号:2-421
  • 获奖情况:
  • 2000年中国科学院优秀期刊一等奖,2000年山东省科委、新闻出版黑署评估优秀级,中国期刊方阵“双百”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:21021