中文摘要：

从生产2，4．滴丁酯的农药厂排污口采集污泥样品，驯化分离得到l株能够以2，4-滴丁酯为唯一碳源和能源生长的细菌12，眩最高耐受2．4．滴丁酯浓度为200mmoL／L。根据其形态特征、生理生化特性、16SrRNAm序列分析，初步鉴定T2为球形赖氨酸芽孢杆菌（Lysinibacillussphaericus）。采用正交试验优化得到T2最适生长条件为：接种量5％，培养基初始pH值为7，培养基装液量为50mL／250mL，培养温度30℃。

英文摘要：

A 2,4-D butyl ester-resistant bacterial strain 32 was screened and isolated from sludge of 2,4-D butyl ester factory outfal]. T2 could use 2,4-D butyl ester as both carbon source and energy source. T2 could endure in 200mmol/L 2,4-D butyl ester concentration. T2 has been tentatively identified as Lysinibacillus sphaericus based on its morphological, physiological and biochemical properties, the results of 16S ribosomal RNA （rRNA） gene se- quence analysis. The optimum growth conditions of T2 were optimized as follows by orthogonal experimental design ： quantity of inoculation with 5%, initial oH value with 7. 0 ,aeration with 50mL/250mL,temperature with 30~C.