通过发芽试验和生物测定方法,研究了乙草胺胁迫对玉米种子萌发特性、幼苗生长发育和生理生化特性的影响。结果表明:浓度为10-100mg/L乙草胺药液胁迫能够降低种子发芽率、发芽势、发芽指数和活力指数,抑制玉米幼苗的根长、芽长和鲜重。同时乙草胺胁迫降低了玉米种子萌发过程中仅.淀粉酶活性和幼根根系活力,导致幼芽和幼根超氧化物歧化酶和过氧化物酶活性上升,对生理环境造成威胁。玉米受抑制程度随着乙草胺浓度的增加而增大,低浓度的乙草胺对玉米的抑制作用较小,浓度超过40mg/L的处理显著影响玉米生长和生理。芽长和根长与乙草胺浓度具有良好的浓度效应关系。
The effects of acetochlor on seed germination, seedling growth and physiological and biochemical characteristics under the concentration stress were investigated through the germination experiment and the bioassay method. The results showed that acetochor solution stress with the concentration of 10 - 100mg/L could reduce the germini- tion percentage, germinition energy, germinition index and vigor index of seed, inhibited the growth of root length, shoot length and fresh weight of seedlings. Acetochor stress reduced a-amylase of seed and root activity of radicle, increased the superoxide dismutase(SOD) activity and the peroxidase (POD) activity in plumule and radicle and caused the physiological injury. The inhibition of maize increased along with the increasement of acetochlor concentration. The inhibition of lower concentration of acetochlor was relatively small and inhibition of concentration of acetochlor (higher than 40mg/L) on growth and physiology was significant. The shoot and shoot length had better negative concentration-effect relationship.