中文摘要：

主要观察了外源生长素长时间（8h）处理对拟南芥生长素极性输出载体PIN2-GFP质膜丰度的影响，低温、KCN处理和生长素受体突变对生长素调控质膜PIN2-GFP丰度的影响，以及液泡H＋-ATPase抑制剂ConA对野生型和生长素受体四突变体tirlafb1，2，3液泡中PIN2-GFP积累的影响．结果表明：外源生长素通过其受体TIRl／AFB介导的信号途径下调质膜PIN2-GFP的丰度，促进PIN2-GFP的内吞、胞内运输和液泡降解．暗示生长素通过TIRl／AFB介导的信号途径反馈调控质膜PIN2-GFP的水平，从而阻止了胞内生长素的过多输出．

英文摘要：

The effects of long-term （ 8 h ） treatments with exogenous auxins on auxin efflux transporter PIN2- GFP plasma membrane （ PM ） abundance, and low temperature, KCN and auxin receptors TIR1/AFBs muta- tion on auxin regulation of PIN2-GFP PM abundance, and ConA, a vacuolar H ~ -ATPase inhibitor, on vacuo- lar accumulation of PIN2-GFP in wild-type and auxin receptor quadruple mutants tirlafol, 2, 3 cells in Arabi- dopsis were systematically studied. The results revealed that exogenous auxins down-regulated PIN2-GFP PM abundance through TIR1/AFB-mediated signaling and promoted PIN2-GFP internalization, intracellular brane trafficking, and vacuolar degradation, indicating that auxin feedback regulates PIN2-GFP PM mem- levels through TIR1/AFB-mediated signaling and inhibits excessive transport of intracellular auxin out of the cell