中文摘要：

目的观察不同信号转导调控剂对严重烫伤小鼠脾脏T淋巴细胞分泌白细胞介素（IL）2、IL-10功能的影响，并探讨其机制。方法分离正常小鼠及高压热蒸气烫伤小鼠（烧伤面积18％TBSA，Ⅲ度）伤后12、96h的脾脏T淋巴细胞。分为正常对照组（不加T淋巴细胞活化相关信号转导分子调控剂）、调控剂组[正常细胞分别加入以下调控剂：膜蛋白激酶C（PKC）抑制剂H-7（50μmoL／L，1ml）、PKC激活剂佛波酯（TPA，30μmol／L，1ml）、非受体型蛋白酪氨酸激酶（PTK）抑制剂Herbimycin（10μmol／L，1ml）、丝裂原活化蛋白激酶激酶（MAPKK）抑制剂PD098059（25μg／ml，1ml）和ca“导人剂A23187（100nmol／L，1ml）]、烫伤组（不加调控剂）、烫伤＋调控剂组（加入上述调控剂）。检测各组细胞IL-2、IL—10的分泌水平。结果伤后各组细胞中IL-2、IL-10含量均低于正常对照组（P〈0．05或0．01）；烫伤12h＋H-7组、烫伤96h＋H-7组分别低于烫伤12h组和烫伤96h组（P〈0．01）。TPA能使IL-2、IL-10分泌明显升高，但对IL-2升高效能更显著。加入PD098059的各组IL-2、IL-10值均低于正常对照组（P〈0．05或0．01）。Herbimycin能显著降低烫伤12h＋Herbimycin组及烫伤96h＋Herbimycin组IL-2的分泌，对IL-10亦有明显抑制。烫伤12h＋A21387组中IL-2、IL—10含量为（2417±39）、（2793±25）pg／ml，烫伤96h＋A21387组为（92l±50）、（2633±35）pg／ml均明显高于烫伤12h组[（1542±40）、（2390±15）pg／ml]和烫伤96h组[（328±19）、（1618±21）pg／，ml（P〈0．05或0．01）]。结论PKC、Ca^2＋、MAPKK和PTK在严重烫伤小鼠脾脏T淋巴细胞分泌IL-2、IL-10的功能紊乱中起重要作用，其中PKC和PTK主要作用于IL-2的分泌而MAPKK影响IL—10的分泌。TPA和A21387均能显著提高烫伤后IL-2的分泌，并明显纠正伤后IL-2／IL-10分泌比值的失调。

英文摘要：

Objective To investigate the influence of various signal transduetion modulators on the splenic T lymphocytes secretion of IL-2 and IL-10 in severely scalded mice, and to explore its mechanism. Methods The mice were inflicted with 18% TBSA full-thickness scald by high-pressure heat vapour, and T lymphoeytes were isolated from murine splenoeytes through nylon wool column at 12 and 96 post-scald hours （ PSH）. Then the cells were divided into following groups： i.e. control, scald, scald and modulator [ 1 ml of 50 μLmol/L PKC inhibitor （H-7）, 30 μLmol/L tetradeeanoylphorbol-13-aeetate （TPA）, 10μLmol/L nonreceptor tyrosine protein kinase inhibitor （hebimyein） , 25 μg/ml of mitogen activated protein kinase kinase inhibitor （PD098059） , 100 nmol/L Calcium ionophore（A23187） were added to the cells, respectively] groups. The scald group was subdivided into S1 （with scald at 12 PSH） and S2 （with scald at 96 PSH） groups. The modulator group was subdivided into modulator, S1 and modulator（the modulators were added into cells at 12 PSH） , and S2 and modulator（ the modulators were added to cells at 96 PSH） groups. The influence of modulators to T lymphocyte secretion of IL-2 and IL-10 were observed. Results After the addition of H-7, the IL-2 and IL-10 levels in each group were obviously lower than that in controls（ P 〈0.05 or 0.01 ） , and that in S1 and H7 group, S2 and H7 group were obviously lower than that in scald group at corresponding time-points（ P 〈 0.01 ）. The levels of IL-10, and especially IL-2 were elevated by TPA, but they were markedly lower than that in control group after PD098059 pretreatment. The secretion of IL-2 and IL-10 was significantly supressed by herbimycin in S1 and herbimycin, and S2 and herbimycin groups,but those in S1 and A21387[（2 417 ±39）pg/ml, （2 793 ±25）pg/ml],S2 and A21387[（921 ±50）pg/ml, （2 633±35 ）pg/ml ] groups were evidently higher than those in S1 [ （1 542± 40）pg/ml, （2 390 ±15 ）pg/ml ],