通过构建双极室微生物燃料电池(microbial fuel cell,MFC),以铁氰化钾溶液为阴极电子受体,以硝基苯(nitrobenzene,NB)和葡萄糖为混合燃料,研究MFC的产电特性和NB的降解情况.结果表明,在外阻为1000Ω的条件下,随着NB初始浓度的增加,双极室MFC的产电特性明显受到抑制.当葡萄糖浓度为1000mg/L,NB初始浓度分别为0、50、150、250mg/L时,MFC的运行周期逐渐缩短,分别为55.7、51.6、45.9、32.2h;最大输出电压分别为670、597、507、489mV;最大体积功率密度分别为28.57、20.42、9.29、8.47W/m3;电荷量分别为65.10、43.50、35.48、30.32C.MFC利用NB和葡萄糖为混合燃料,可以在稳定地输出电能的同时实现有机物高效降解,MFC对NB去除率高达100%,对COD的去除率达到87%~98%.但以250mg/LNB为单一燃料时,MFC无明显产电现象.DGGE图谱表明NB的加入改变了MFC阳极电极上微生物的群落结构.
By constructing a dual-chamber microbial fuel cell(MFC),experiments were carried out using an initial glucose concentration of 1 000 mg /L with different nitrobenzene(NB) concentrations(0,50,150 and 250 mg /L) as the MFC's fuel.Results showed that with an external resistance of 1 000 Ω,the initial glucose concentration of 1 000 mg /L and the initial NB concentrations of 0,50,150,250 mg /L,the operation periods were 55.7,51.6,45.9 and 32.2 h,respectively,the maximum voltage outputs were 670,597,507,and 489 mV,the maximum volumetric power densities were 28.57,20.42,9.29,and 8.47 W /m3,and the electric charges were 65.10,43.50,35.48,and 30.32 C.The MFC could use the NB and glucose mixtures as fuel and generated stable electricity outputs.The degradation rates of NB in the MFC in all cases reached up to 100% and COD removals in the MFC were 87% 98%.However,the electricity generation was negligible when using 250 mg /L NB as the sole fuel.Denaturing gradient gel electrophoresis(DGGE) profiles demonstrated that the presence of NB resulted in changes of the dominant bacterial species on the electrodes.