中文摘要：

为了探讨大豆低聚糖（SBOS）改善肉鸭肠道微生物群落结构的效果及机理,本试验运用末端限制性片段长度多态性（T-RFLP）方法研究了SBOS对樱桃谷鸭盲肠内容物中大肠杆菌、双歧杆菌和乳酸杆菌数量的影响。结果表明：与对照组相比,SBOS降低了盲肠内大肠杆菌的数量,其中第3、4组（添加300和500mg·kg-1SBOS）在第3、4周达到显著水平（P〈0.05）,第5组（添加700mg·kg-1SBOS）在第3周达到显著水平（P〈0.05）;SBOS对盲肠内双歧杆菌和乳酸杆菌有明显的增殖作用,其中在第3、4周,第4组2种菌的增殖达到显著水平（P〈0.05）;添加SBOS对于调节鸭肠道微生物群落效果与添加金霉素（100mg·kg-1）的效果差异不显著。SBOS能有效调节樱桃谷鸭肠道微生物群落结构,且呈剂量和时间依赖效应,本试验中,500mg·kg-1是最适添加量。

英文摘要：

In order to understand the effects of soybean oligosaccharide （SBOS） on the intestinal microbial community structure in Cherry Valley ducks,T-RFLP method was adopted to study the amounts of Escherichia coli,Bifidobacterium,and Lactobacillus in the duck caecum under effects of different concentration SBOS.Applying SBOS decreased the amount of E.coli in caecum.Compared with that of the control,the amount of E.coli in the 3rd and 4th week after applying 300 and 500 mg·kg-1 of SBOS and in the 3rd weed after applying 700 mg·kg-1 of SBOS had a significant decrease （P0.05）.Applying SBOS promoted the proliferation of Bifidobacterium and Lactobacillus,being significantly （P0.05） in the 3rd and 4th weeks after applying 500 mg·kg-1 of SBOS.No significant differences were observed between applying SBOS and aureomycin in the effect of regulating microbial community structure in duck intestine.Our results indicated that SBOS could effectively regulate the microbial community structure in Cherry Valley duck intestine,the effect was time- and dose-dependent,and the optimal additive dose was 500 mg·kg-1.