中文摘要：

目的探讨IL-2、IL-6基因SNPs与房颤发生的关联。方法选取2012年1～6月医院收治的房颤患者80例为观察组，随即选择同期在门诊健康体检人员60名为对照组，采集两组外周血检测基因DNA，用PCR方法扩增1L-2基因包含突变位点-330的片段以及IL-6基因包含突变位点-572的片段，采用PHASE10软件构建这两个多态性位点的个体单倍体型；以非条件logitic回归校正混杂因素，采用SPSS软件进行多态性与房颤风险关联性的统计分析。结果两个多态性位点在部分人群中具有多态性，两组人群中-330（IL2）及-572（IL6）多态性位点差异有统计学意义（P〈0．05）；IL-2基因-330位点和IL-6基因的-572位点多态性对于房颤的发生有着显著的易患关联，IL-2基因～330位点杂合、纯合形式分别增加房颤患病风险1．46倍和3．63倍；IL-6基因的～572位点杂合、纯合形式分别增加房颤患病风险1．32倍和1．36倍；logistic回归分析结果表明，携带TL-2的～330T／G位点突变基因G并且同时携带IL-6—572C／G位点的突变纯合子TT／GG能增加房颤患病风险（OR-1．22，95％CI-0．68～2．69）；IL-2的-330T／G和IL-6的-572C／G位点突变存在左房直径水平差异。结论IL-2基因-330T／G和IL6～572C／G位点多态性存在交互作用，基因基因交互作用可能增加房颤发生风险。

英文摘要：

OBJECTIVE To explore the correlation between the atrial fibrillation （AF） and the single nueleotide polymorphisms （SNPs） of the IL-2 and II.-6. METHODS A total of 80 cases of AF who were treated in the hospital from Jan to Jun 2012 were chosen as the observation group, while 60 healthy people who took physical examination in the outpatient department were set as the control group, then the peripheral blood specimens were sampled for DNA test, the frequency distributions of genotypes and alleles of the IL-2- 330T/G and IL-6- 572C/G were amplified by polymerase-chain-reaetion-restrietion fragment length polymorphism （PCR-RFLP）, the PHASE10 software was employed to establish individual haplotypes of the two SNPs, the confounding factors were corrected through non-conditional logistic regression, and the statistical analysis were performed for the relationship between the polymorphisms and the atrial fibrillation with the use of SPSS software. RESULTS The two polymorphic loci posed polymorphism in part of the population, and the difference in the IL-2 -- 330T/G between the two groups was statistically significant（P〈0.05）; the difference in the IL-6- 572 C/G was statistically significant （P〈0.05） ; the IL-2-- 330T/G and the IL-6-- 572 C/G were significantly correlated with the incidence of AF. The heterozygous IL-2--330T/G increased 1.46 times the risk of AF, and the homozygous IL-2 --330T/G in- creased 3.63 times the risk of AF; the heterozygous IL-6--572 C/G increased 1.32 times the risk of AF, and the homozygous IL-6--572 C/G increased 1.36 times the risk of AF. The result of logistic regression analysis indica- ted that the carrier of mutation gene G of IL-2--330T/G and the homozygous TT/GG of IL-6--572 C/G could in- crease the risk of AF （OR=1. 22, 95%CI=0.68-2.69） ; there was difference in the left atrial diameter between the IL-S--330T/G and the IL-6--572 C/G. CONCLUSION There are interactions between polymorphisms of IL-2 --330T/G and IL-6--572C/G, and the gene gene interaction may