中文摘要：

从农药厂废水中分离到一株降解莠去津的节杆菌（Arthrobacter sp.）HB-5，以从该菌中提取到的降解酶对莠去津的降解率为指标，进行最佳产酶培养基及发酵条件的优化研究，对其产酶量进行了评价,通过正交试验和均匀试验，对细菌HB-5的发酵培养基进行了优化研究,运用SAS软件进行结果分析，所获优化培养基配方为：蔗糖3.0g·L^-1，莠去津0.38g·L^-1，K2HPO_40.5g·L^-1，KH_2PO_41.2g·L^-1，MgSO_4·7H_2O1.2g·L^-1，Nacl 0.1g·L^-1，微量元素溶液3.8mL·L^-1得到菌株培养的最佳优化条件为：菌株发酵液培养时间为48h，接种量为2％，发酵液初始pH值为9，250mL三角瓶中装液量为80mL,经优化后，降解酶对莠去津的降解率（91.64％）比原培养基（40.67％）提高了125％。

英文摘要：

An atrazine degrading bacterium （Arthrobacter sp.） HB-5, was isolated in our laboratory from pesticide waste water. The optimum fermentation conditions and fermentation culture medium of HB-5 were selected by uniform experimental design and orthogonal experimentation, and the amount of enzyme produced by HB-5 was estimated from the atrazine degradation rate. The experimental results were analyzed by the SAS system. The ideal composition of culture medium of HB-5 included saccharose 3.0 g·L^-1, atrazine 0.38 g·L^-1, K_2 HPO_4 1.2 g·L^-1, K_2 HPO_4 0.5 g·L^-1, MgSO_4·7 H_2O 1.2 g·L^-1, NaCl 0.1 g·L^-1, microelements 3.8 mL·L^-1. The optimum culture conditions of HB-5 were ： culture time 48 h, inoculum size 2% , initial pH 9.0 and amount of culture medium 80 mL. The degradation rate of atrazine by the optimum fermentation liquid reached up to 91.64% , and increased 125% over the original culture medium （40.67%）.