中文摘要：

依据IFNα1和THYα1的分子结构特征设计了1个连接肽，根据氨基酸序列和大肠杆菌密码子的偏爱性，采用PCR方法合成了三者融合基因，将该基因克隆至pGEX4T-2载体中，构建了pGEX4T-2-IFNα1-THYα1原核表达质粒。将重组质粒转化至BL21（DE3）受体菌中，37℃培养至D600为0．6～1．0时，IPTG（1．0mmol／L）诱导培养4h，经SDS-PEGA和Western-blot鉴定，目的蛋白获得高效表达。

英文摘要：

Interferon α1 and thymosin α1 are both important cytokines in immunoloregulation. Clinical researches showed that combination use of interferon α1 and thymosin al could enhance the biological function in the treatment of virosis. To identify whether the fusion protein of these two cytokines have more effective biological functions,the fusion gene of interferon α1/ thymosin α1 was linked by a flexible oligopeptide gene and cloned into pGEX4T-2 expression plasmid. The recombination plasmid was transformed to BL21 engineering bacteria. SDS-PAGE and Western-blot results illustrated that the fusion protein was expresssd in E. coli strain BL21 （DE3）.