中文摘要：

根据GenBank已发表的鸡干扰素α1（IFNα1）基因序列．设计1对特异性引物，采用PCR方法，从鸡基因组中扩增出鸡IFNα1基因。根据胸腺肽α1（THYα1）氨基酸序列，选用大肠杆菌偏爱密码子．设计基因序列，同时在IFNα1-THYα1之间设计1个5肽Linker。在IFNα1基因结构基础上．设计4条寡核苷酸单链作为下游引物．以搭桥的方式延伸和扩增，最终获得IFNα1-THYα1融合基因；将该基因克隆至pGEX4T-1载体后，转化至BL21（DE3）受体菌中，37℃培养至菌液D600为0．6～1．0时，IPTG（1．0mmol／L）诱导培养4h；经western Blot鏊定证实，成功构建了鸡干扰素α1及胸腺肽α1融合蛋白原核表达系统，表达产物以包涵体形式存在．获得高纯度的IFNα1—THYα1蛋白。以细胞痛变抑制试验及E玫瑰花结形成试验对目的蛋白进行体外活性检测．结果显示该蛋白具有生物活性。

英文摘要：

Accoding to the published genic sequences of chicken interferon-α1 ,a pair of specific primers was de signed to amplified the genc of chicken interferon-α1 by polymerase chain reaction （PCR） from the complete genome of chicken. Based on IFN α1 amino acid sequence and the choice of E. coli preference for the codon, we designed a linker of 5 peptides between IFN aland THY α1 and four oligonucleotide single chains as downszream primers. The IFN α1-THY α1 fusion gene was ohlained by PCR. The fusion gene was cloned into pGEX1T -1 expression plasmid and then transformed into BL21（DE3）,cultured in liquid LB at 37℃ and induced with IPTG for 4 hours while its D600 got up to 0.6-1.0. Western blot results indicated that the chicken IFNα1 -THYα1 fusion protein have been successfully expressed and the product of expression exists as the form of inclusion. The high-purity of IFNα1 THYα1 protein was obtained and the activity in vitro of the purposed protein was detected with the cytopathogenic effect inhibition test and E rosette forming assay. The results proved that the protein possess the biological activity.