中文摘要：

目的探讨丙型肝炎病毒（hepatitis C virus,HCV）F蛋白与多发性腺瘤样息肉病（adenomatous polyposis coli,APC）基因甲基化在慢性HCV感染中的关系。方法收集慢性HCV患者（chronic hepatitis patient,CHP）的血样,检测F抗体（F antibody,F-Ab）的阳性率并且分成两组（F-Ab（＋）CHP组、F-Ab（-）CHP组）,收集健康者的血样作为对照组;分离外周血单核细胞（peripheral blood mononuclear cells,PBMCs）,分别用HCV Core/F蛋白刺激,72 h后,分别提取DNA,用二代测序检测APC基因的甲基化水平,分析各组APC基因甲基化程度的差异。结果APC基因甲基化程度在F-Ab（＋）CHP组、F-Ab（-）CHP组及健康对照组三组间差异有统计学意义（F=185.185,P〈0.001）,F-Ab（＋）CHP组APC基因甲基化程度高于F-Ab（-）CHP组,健康对照组最低（均有P〈0.05）;三组样本PBMC分别经Core/F蛋白体外刺激后,APC基因甲基化程度各组总体比较差异均有统计学意义（均有P〈0.05）,Core蛋白与F蛋白共刺激组细胞APC基因甲基化程度高于F蛋白刺激组,Core蛋白刺激组为最低。结论在慢性HCV感染患者中,HCV F蛋白的产生能够影响APC基因甲基化程度。

英文摘要：

Objective To investigate the relationship between F protein and the methylation of adenomatous polyposis coli（ APC） gene in chronic hepatitis C virus（ HCV） infection. Methods Blood samples collected from chronic HCV patients（ CHP） were divided into F-Ab（ ＋） CHP group and F-Ab（-） CHP group,blood samples from healthy persons were used as control. Isolated peripheral blood mononuclear cells（ PBMCs） were stimulated by Core protein and F protein for 72 hours,and then cell DNA was etracted to detect the methylation level of APC gene. Results The methylation level of APC gene in three groups was significantly different（ F = 185. 185,P〈0. 001）. The methylation level of APC gene in FAb（ ＋） CHP group was significantly higher than that in F-Ab（-） CHP group,the healthy control group was the lowest（ all P〈0. 05）. There was significant difference in methylation level of APC gene by Core/F protein stimulated in vitro in all subgroups（ all P〈0. 05）. The methylation of APC gene in cells stimulated by Core protein and F protein was higher than that in cells stimulated by F protein,which was higher than that in cells stimulated by Core protein. Conclusions HCV F protein can affect the methylation of APC gene in chronic HCV infection.