中文摘要：

目的建立不同来源板蓝根药材乙酸乙酯提取物特征指纹图谱。方法采用反相高效液相色谱法，ODS-C18色谱柱（250mm×4．6mm，5μm．），流动相为甲醇-0．2％磷酸水溶液系统，线性梯度洗脱，检测波长230nm，进样量为10μl。实验测定了10批药材并记录了指纹图谱，不同样品之间的相似度采用国家药典委员会开发的中药指纹图谱相似度评价系统（2004A）计算。结果建立了板蓝根药材乙酸乙酯提取物HPLC指纹图谱，以峰形较好、峰面积较高的3号峰为参照峰，确立了板蓝根药材乙酸乙酯提取物指纹图谱的14个特征共有峰，但特征峰的相对含量差异较大，导致指纹概貌差异。结论所建立的HPLC指纹图谱有很好的精密度、重现性和稳定性，为深入研究板蓝根的质量标准提供了实验基础。

英文摘要：

Aim To establish the HPLC fingerprints of EtOAc extraction of Radix Isatidis from various sources. Methods Fingerprints of EtOAc extraction from 10 batches of Radix Isatidis from various sources were established by a High Performance Liquid Chromatography （HPLC） method based on a Kromasil C18 column （250mm ×4.6mm,5μm） with a gradient elution solvent system composed of methanol-0.2% H3PO4 water solution; the detection wavelength was set at 230nm;sample injection was 10μl. The fingerprints were compared with similarity evaluation software published by the Committee of China Codex. Results HPLC Fingerprints of EtOAc extraction of Radix lsatidis were established. 14 common peaks were identified as the characteristic fingerprints of Radix Isatidis. Peak 3 with fine peak form and large peak area was selected as the reference peak. However, the content of each peak showed great difference among the samples, which resulted in the difference of their chromatograms. Conclusion The established HPLC fingerprints have desirable precision, reproducibility, stability, providing an experimental basis for further quality control of Radix Isatidis.