中文摘要：

目的探讨TGF-β1对体外培养的星形胶质细胞增殖的影响及其Smad3信号转导机制。方法体外培养的原代星形胶质细胞,传代接种后采用SiRNA基因沉默技术,干扰Smad3基因的表达,RT-PCR验证沉默效果,应用免疫荧光技术观察干扰TGF-β1-Smad3通路后对星形胶质细胞增殖的影响。结果 TGF-β1对星形胶质细胞增殖有明显促进作用,并呈现一定的剂量依赖性;成功沉默Smad3基因后,星形胶质细胞的增殖率明显减低;成功沉默Smad3基因能干扰TGF-β1对星形胶质细胞增殖的促进作用。结论 TGF-β1-Smad3信号转导通路对星形胶质细胞增殖起调节作用。

英文摘要：

Aim To investigate the effect of TGF-β1 on astrocyte proliferation and explore the possible mechanism involving Smad3 transduction.Methods Astrocytes were derived from newborn mice and then subcultures.RNA interference technique was used to interfere with Smad3 gene expression.RT-PCR was conducted to determine the transfection efficiency.Results TGF-β1 significantly promoted the proliferation rate of astrocyte as compared with the control group in a dose-dependent manner.While Smad3 gene was successfully interfered,Smad3-SiRNA attenuated astrocyte proliferation and suppressed the facilitation of astrocyte proliferation induced by TGF-β1.Conclusion TGF-β1-Smad3 pathway is involved in the regulation of astrocyte proliferation.