中文摘要：

为了初探自制金英黄归液对金黄色葡萄球菌（S．aureus）的抗菌作用，作者常规制备金英黄归注射液，采用试管二倍稀释法测定其对S．aureus的最小抑菌浓度（MIC）和最小杀菌浓度（MBC），通过S．aureus人工感染小鼠试验研究其在体内的抑菌作用，在MBC下应用电镜观察其对细菌形态结构的影响，采用比色法研究其对细菌的琥珀酸脱氢酶（SDH）比活性的影响。结果表明金英黄归液对s．aureus的MIC和MBC分别为31．2和62．5nag·mL，能显著抑制S．aureus引起的小鼠死亡（P〈0．05），在MBC作用下的S．aureus细胞壁上有大量絮状物，细胞呈塌陷状，甚至细胞壁破裂，能极显著降低细菌体内SDH比活性（P〈0．01）。结果提示金英黄归液对S．aureus具有抑菌作用，抑菌机制是破坏细菌的细胞壁，抑制菌体内的酶系统。

英文摘要：

The aim of this study was to study antibacterial effects of Jin-Ying-Huang-Gui Injection （JYHGI）, a Chinese herbal formula, on Staphylococcus aureus （S. aureus）. JYHGI was pre- pared by routine method. The minimal inhibitory concentration （MIC） and minimal bactericidal concentration （MBC） of S. aureus were detected with tube double dilution method in vitro. The mice model was prepared by vena caudalis injection of S. aureus to carry antibacterial experiment in vivo. In addition, we also studied the influence of JYHGI on ultrastructure and the specific ac- tivity of succinate dehydrogenase （SDH） of S. aureus. Antibacterial activity showed that the MIC and MBC of JYHGI to S. aureus were 31.2, 62.5 mg . mL 1, respectively. Mortality of mice in JYHGI could significantly decrease mice mortality caused by S. aureus （P〈0. 05）. The JYHGI treated cell wall of S. aureus were covered with a large number of floc, the cells seemed to be collapsed-like, some cell wall even ruptured. The SDH specific activity of JYHGI group were significantly lower than that of control group （P〈0.01）. It is concluded that the inhibition mechanism is that JYHGI could damage the cell wall and inhibit the enzyme system of S. aureus.