中文摘要：

靶向肿瘤细胞代谢过程中关键调控分子抑制肿瘤细胞生长的研究日益成为热点。目前,研究肿瘤细胞氧化磷酸化和有氧糖酵解主要是应用Clark氧电极法测定细胞氧耗率以及对相关中间代谢物的测定,如乳酸和葡萄糖。但是,这些方法测定的指标相对单一,而且过程繁琐。该文详细介绍了生物能量分析仪在研究肿瘤细胞糖酵解和线粒体氧耗率中的应用,并通过研究肿瘤细胞应用阿霉素及相关药物处理后生物能量代谢的变化,深入探讨了这一方法在研究肿瘤细胞生物能量代谢方面中的优越性。研究结果表明,羰基氰–对–三氟甲氧基本腙（carbonylcyanide p-trifluoro methoxyphenylhydrazone,FCCP）的浓度以及细胞数量对于研究肿瘤细胞的氧耗率十分关键,应用阿霉素能够显著抑制肿瘤细胞的有氧糖酵解和线粒体氧耗率。通过该文的介绍,期望能为肿瘤细胞生物能量代谢研究提供进一步的参考。

英文摘要：

In recent years, growing studies are focusing on cancer therapy via targeting key molecules in cancer cell metabolism. The traditional methods for the study of oxidative phosphorylation and aerobic glycolysis of cancer cells are oxygen consumption rate（OCR） measurement by Clark oxygen electrode method and the determination of crucial inter-mediate substrates, such as L-lactate and glucose. However, the traditional methods used to determine the variation of cell bioenergetics are very complicated and time consuming. Here, we introduced a novel method which works effectively in the study of cellular bioenergetics and investigated the cellular bioenergetics of cancer cells with or without doxorubicin and certain drugs pretreatment to demonstrate the advantage of Extracellular Flux Analyzer. Our data indicated that the concentration of carbonylcyanide p-trifluoromethoxyphenylhydrazone（FCCP） and the cell density are crucial for the oxygen consumption detection. Additionally, our results showed that doxorubicin inhibits tumor cell growth may also through disrupting aerobic glycolysis and mitochondrial respiration. Overall, our work may provide reference for the investigation of cellular bioenergetics.