中文摘要：

目的：探讨姜黄素对四氯化碳（carbon tetrachloride,CCl4）诱导的大鼠肝纤维化的影响及姜黄素能否抑制肝脏NADPH 氧化酶4（NADPH oxidase 4,NOX4）的表达.方法：腹腔注射CCl4（每周2次,共6周）诱导SD大鼠肝纤维化模型.将SD大鼠随机分成4组：正常对照组（n=10）、单纯给药组（n=10）、模型对照组（n=15）和姜黄素预防组（n=1 5）,姜黄素给药剂量为200 mg/kg体质量.检测血清中ALT、AST及活性氧自由基（reactive oxygen species,ROS）的水平;肝损伤和肝纤维化评估采用肝组织HE染色与天狼星红染色;分别用实时荧光定量PCR、Western blotting和免疫组织化学方法检测肝组织NOX4的表达.结果：姜黄素能减轻CCl4诱导后大鼠的肝损伤与肝纤维化,抑制血清ROS的产生.与模型对照组相比,姜黄素预防组的肝纤维化大鼠肝脏中NOX4 mRNA与蛋白表达水平显著降低.结论：姜黄素能减轻CCl4诱导大鼠发生的肝损伤与肝纤维化,其效应可能与抑制肝脏NOX4过表达有关.

英文摘要：

Objective：The aim of this study was to investigate the effect of curcumin on liver fibrosis and hepatic expression of NADPH oxidase 4 （NOX4） in rat model.Methods：The SD rats were intraperitoneally injected with carbon tetrachloride （CCl4）twice a week for 6 weeks to induce liver fibrosis.The rats were randomly divided into four groups：normal control group （n =10）,curcumin-treated control group （n =10）,model control group （n =15） and curcumin prevention group （n =15）.Curcumin was given at a dose of 200 mg/kg body weight daily.Serum transaminase activity and reactive oxygen species （ROS） were assessed.Hematoxylin-eosin （H＆E） staining and sirius red staining were performed to evaluate liver injury and fibrosis.Moreover,the levels of NOX4 mRNA and protein expression were determined by real time-PCR,Western blotting and immunohis tochemistry.Results：Chronic CCl4 treatment caused liver injury,ROS increase,and collagen accumulation in the liver.Treatment with curcumin could significantly reduce liver injury,fibrosis and ROS.Furthermore,curcumin significantly attenuated the expression of NOX4 in liver.Conclusions：Curcumin significantly attenuates the severity of CCl4-induced liver injury and fibrosis,and the beneficial effects may be partly through inhibition of hepatic NOX4 expression.