采用盆栽试验方法,在模拟Pb和Zn胁迫(1000mg·kg-1)条件下,研究不同用量的氮肥(0、0.1、0.3g·kg-1和0.5g·kg-1)处理对小花南芥(Arabis alpinal var.parviflora Franch)生长、土壤pH值、土壤Pb和Zn的不同形态以及小花南芥的生物量、叶绿素、脯氨酸、质膜透性、Pb和Zn累积特征的影响。结果表明:施用氮肥导致土壤pH值呈下降趋势,施N导致土壤中Pb水溶态和可交换态含量从3.9%显著增加到11.5%~13.7%,Zn水溶态和可交换态含量从13.4%显著增加到19.3%~23.5%。施N处理使小花南芥叶绿素含量和生物量显著增加。低N(0.1~0.3g·kg^-1)处理时,小花南芥叶片脯氨酸含量和相对电导率上升;小花南芥地上部和地下部Pb和Zn含量和累积量随着N素的施入而增加。施N(0.1~0.3g·kg^-1)处理时,Pb和Zn的富集系数、转移系数均大于1。因此,适量施N(0.1g·kg^-1)能促进小花南芥对土壤中Pb和Zn的吸收和提高小花南芥对Pb和Zn污染土壤的修复效率。
Pot experiments were conducted to study the effects of N application on soil pH value, forms of Pb and Zn, biomass, contents of chlorophyll and praline, membrane permeability, and accumulation characteristics of Pb and Zn under Pb and Zn stress(1000 mg·kg-1 ). The results showed that soil pH value decreased with N application. Percentage of bio-available Pb and Zn in soil reached the maximum under N 0.1 g·kg-1 and 0.3 g·kg-1 comparing with other treatments, respectively. With N application, water soluble Pb contents and exchangeable Pb contents increased from 3.9% to 11.5% ~13.7% and water soluble Zn contents and exchangeable Zn contents increased from 13.4% to 19.3%~23.5%. Chlorophyll contents and biomass increased with increase in rates of N application. Praline content and membrane permeability in leaves of A. alpinal increased with N 0.1~0.3 g·kg-1 application. Contents and accumulation of Pb and Zn in shoot and root of A. alpinal increased with increase in rates of N application. Bioaccumulation factor and transfer factor of Pb and Zn were more than 1. There- fore, adsorption of Pb and Zn of A. alpinal should be promoted and remediation efficiency of A. alpinal to soil contaminated by Pb and Zn could be improved by suitable N application(0.1 g·kg^-1 ).