为探讨续断菊/玉米间作种植模式对Cd污染的响应机制,通过盆栽实验,研究了不同质量分数的Cd(0,50,100,200 mg/kg)对玉米与续断菊间作体系下植物叶片可溶性蛋白、丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和总抗氧化酶(T-AOC)活性的影响。结果表明:在0和50 mg/kg Cd胁迫条件下,间作玉米和续断菊叶片可溶性蛋白含量降低了8.1%~34.1%,当Cd处理质量分数为100和200 mg/kg时,间作玉米叶片和续断菊叶片可溶性蛋白显著比单作增加了34%~69.1%;间作玉米叶片MDA含量较单作增加了6.1%~64.6%,续断菊叶片MDA含量较单作降低了9.7%~58.7%;在不同Cd质量分数处理下,无论是单作还是间作,玉米和续断菊叶片中SOD、POD、CAT和T-AOC活性均随Cd质量分数的增加而增加,仅Cd质量分数达到200 mg/kg时CAT活性有所下降;同一Cd质量分数处理下,间作玉米叶片SOD、POD(200 mg/kg除外)、CAT和T-AOC活性均较单作降低,分别较低了15.1%~48.3%、2.8%~24.7%、5.2%~42.3%和20.7%~31.9%;间作续断菊叶片T-AOC较单作增加了11.2%~26.4%。总体上,在一定质量分数的Cd胁迫下,玉米和续断菊叶中的MDA含量及抗氧化酶活性均有所提高以维持正常生长,表明抗氧化酶在清除Cd毒害产生的活性氧自由基和提高Cd耐性中有重要作用。
Intercropping has shown the potential to phytoremediate heavy metal polluted soils. A pot experiments were carried out to explore the antioxidant responses of Sonchus asper L. Hill and Zea mays L. to Cd pollution under intercropping system. Four cadmium levels( 0,50,100,200 mg / kg)was designed to study the effects of Cd on the contents of soluble protein and MDA,activities of SOD,POD,CAT and T-AOC in leaves of S. asper and Z. mays. The result showed that: with 0 and 50 mg /kg soil Cd content,the content of soluble protein in S. asper and Z. mays decreased by 8. 1% ~34. 1% under intercropping,and with 100 and 200 mg / kg soil Cd content,the content of soluble pro-tein in S. asper and Z. mays significantly increased by 34% ~ 69. 1%. Compared with monoculture,MDA contents of Z. mays increased by 6. 1% ~ 64. 6% and decreased by 9. 7% ~ 58. 7% in S. asper under intercropping. Under both monoculture and intercropping systems,the SOD,POD,CAT,TAOC activities of S. asper and Z. mays all increased with increasing Cd concentrations. But Cd addition at 200 mg / kg caused CAT activity decrease. Under the same Cd concentration treatment,SOD activity,POD activity( except 200 mg / kg),CAT activity and T-AOC activity of Z. mays decreased by15. 1% ~ 48. 3%,2. 8% ~ 24. 7%,5. 2% ~ 42. 3% and 20. 7% ~ 31. 9% in intercropping,compared with monoculture. Under intercropping,T-AOC activity of S. asper increased by 11. 2% ~26. 4%. In general,intercropping could improve antioxidant enzymatic system in Z. mays and S. asper,thus alleviate Cd toxicity under Cd stresses. The enzymatic antioxidation capacity plays important role in Cd tolerance of plant.