中文摘要：

目的：建立脂多糖诱导的急性肺损伤模型。方法：随机将60只昆明种小鼠分为5组,分别向小鼠尾静脉注射生理盐水和不同剂量的脂多糖,12h后检查并评估各组小鼠的急性肺损伤情况,以确定合适的建模脂多糖剂量。确定合适的脂多糖剂量后,采用ELISA法分别于2、6、12h末摘取小鼠眼球取血并分离血清,测定血清中hs-CRP、IL-1β、TNF-α水平;采用HE染色观察小鼠肺脏病理形态变化。结果：脂多糖剂量为4mg/kg时,小鼠血清hs-CRP、IL-1β、TNF-α可持续保持较高水平。HE染色后,AIL组小鼠光镜下可见以肺泡正常结构消失、肺泡间隔明显增厚、肺泡腔内出血、大量炎性细胞浸润为主要表现的组织病理变化,肺损伤6h最为明显。结论：向小鼠尾静脉注射脂多糖4mg/kg可成功复制AIL小鼠动物模型。

英文摘要：

Objective：To establish acute lung injury（ALI）model induced by liPopolysaccharide in mice.Methods：Kun-ming mice were injected respectively with isometric stroke-physiological saline solution and LPS,An evaluation on acute lung injury was been executed at the end of 12 hto confirmed anappropriate LPS dose for establishing mice model.The mice were culled eye ball after injected proper dose of LPS for 2h,6h,12 h.The levels of serum high-sensitivity C-reactive protein（hs-CRP）,tumor necrosis factor alpha（TNF-α）,interleukin-1β（IL-1β）and were detected by enzyme-linked immunosorbent assay.Regular HE staining was applied to evaluate the inflammatory reaction of lung tissue.Results：The level of mice serum IL-1β,TNF-αand hs-CRP were raised after injection of LPS 4mg/kg.By microscopy,the lung tissue strueture of mices in ALI group were damaged,a large mount of neutrophil recruitment and the thiekened interalveolar septum with signifieantly hyperemia in lung tissue of the LPS group.Such changes listed above appeared most severely at the time Point of 6hafter the injection of LPS.The ALI pathological score of the mices of the LPS group was higher than that of NS group.Conclusion：The ALI mice model could be duplicated successfully infusing by injection of LPS 4mg/kg in caudal vein.