中文摘要：

[目的]确定猕猴桃根多糖的纯化工艺及其参数,制备高纯度RACP。[方法]以吸附率和解析率为指标,选定适合猕猴桃根多糖纯化的树脂;采用单因素试验法优化纯化工艺参数。[结果]选定D900型阴离子交换树脂纯化猕猴桃根多糖,其纯化工艺条件为:D900型阴离子交换树脂柱(高4cm,直径2cm),上样浓度(RACP)为8mg/mL,pH值=8,上样体积为2 BV,上柱速度0.4mL/min,柱温为33℃;用5BV 0.6mol/L的NaCl溶液洗脱,洗脱速度为0.8mL/min,收集洗脱液,洗脱液超滤脱盐。采用该工艺条件纯化猕猴桃根多糖,其纯度可达到85%以上。[结论]建立的RACP纯化工艺简单、可行。

英文摘要：

[Objective] To determine the technology of RACP purification and its parameters,and prepare high purity RACP.[Method ] To choose one Anion exchange gum which is adequate for purification of RACP with the index of adsorption rate and analytic rate.And optimize the technology of D900 Anion exchange gum purification of RACP and its parameters by haplo-factor method.[ Results ] To choose D900 Anion exchange gum to purify RACP,and these are its conditions of purification technology :D900 Anion exchange gum(Heigh...