中文摘要：

蛋白质酷氨酸 kinase 侦探，一种非受体酷氨酸 kinase，首先被发现在 T 房间， B 房间，造血的房间，和肝房间被表示为细胞质的蛋白质。侦探被证明了是表明小径的 T 房间受体的一个批评调节的人。在现在的学习，侦探的一个相当或相同的事物在七鳃鳗被识别， Lampetra 装饰用的梨树。L 的全身的侦探 cDNA。装饰用的梨树(Lja 侦探) 包含 1923 bp 编码 641-amino 酸蛋白质的开的读框架。有典型脊椎动物侦探的 Lja 侦探的推出的氨基酸顺序的多排列证明它拥有侦探家庭蛋白质的所有保存领域，显示侦探的 ortholog 在现存无颚的脊椎动物存在。在种系发生的树上，那与 24 个相当或相同的事物被重建无颚并且 jawed 脊椎动物，从七鳃鳗和丑鱼的侦探作为单个 clade 被聚类。在外集团和 agnathan 侦探组之间的基因距离在外集团和 gnathostome 侦探组之间是比那靠近的，显示它的起源比任何 jawed 脊椎动物早远。在像淋巴细胞的房间和鳃的 Lja 侦探的 mRNA 层次被即时量的聚合酶链反应检测。结果证明它是显著地在有混合病原体的刺激下面的 upregulated。这结果被西方的污点分析进一步证实。所有这些结果显示 Lja 侦探在有免疫力的反应起一个重要作用。我们的数据将在无颚的脊椎动物为七鳃鳗侦探和它的免疫学的函数的进一步的学习提供引用。

英文摘要：

Protein tyrosine kinase Tec, a kind of non-receptor tyrosine kinase, is primarily found to be expressed in T cells, B cells, hematopoietic cells, and liver cells as a cytoplasmic protein. Tec has been proved to be a critical modulator of T cell receptor signaling pathway. In the present study, a homolog of Tec was identified in the lamprey, Lampetra japonica. The full-length Tec cDNA of L. japonica （Lja-Tec） contains a 1923 bp open reading frame that encodes a 641-amino acid protein. The multi-alignment of the deduced amino acid sequence of Lja-Tec with typical vertebrate Tecs showed that it possesses all conserved domains of the Tec family proteins, indicating that an ortholog of Tec exists in the ex- tant jawless vertebrate. In the phylogenetic tree that was reconstructed with 24 homologs of jawless and jawed vertebrates, the Tecs from lampreys and hagfish were clustered as a single clade. The gen- etic distance between the outgroup and agnathan Tecs＇ group is closer than that between outgroup and gnathostome Tecs＇ group, indicating that its origin was far earlier than any of the jawed verte- brates. The mRNA levels of Lja-Tec in lymphocyte-like cells and gills were detected by real-time quantitative polymerase chain reaction. Results showed that it was significantly upregulated under stimulation with mixed pathogens. This result was further confirmed by western blot analysis. All these results indicated that Lja-Tec plays an important role in immune response. Our data will provide a reference for the further study of lamprey Tec and its immunological function in jawless vertebrates.