中文摘要：

目的探讨氨肽酶N抑制剂Bestatin对肿瘤患者外周血CD4^＋CD25^＋Treg的生物学效应及机理。方法将Bestatin加入PHA刺激新鲜分离的卵巢癌患者外周血Treg细胞的体外培养体系，采用流式细胞术分析Foxp3和CTLA-4的表达。进而分析Bestatin对Treg细胞抑制CD4^＋CD25T细胞活化增殖的影响。结果Bestatin可抑制Treg胞表达Foxp3和CTLA-4，并促进Treg细胞和CD4^＋CD25^＋T细胞的体外共培养体系中T细胞的增殖。结论抑制氨肽酶N可下调Treg细胞的免疫抑制作用，对肿瘤治疗具有潜在应用价值。

英文摘要：

Objective To explore the biological effect of Bestatin,CD13 specific inhibitor, and its molecular mechanism on CD4^＋CD25^＋Treg cells.Methods Bestatin was added to stimulate the T cells that isolated from peripheral blood of ovarian cancer patients and activated by PHA.T cell proliferation was determined by cell counting at 3d. The CTLA-4 expression of Treg cells was determined by FACS,and Foxp3 analysis was performed after fixation and intracellular staining.Results Bestatin could decrease the expression of CTLA-4 and Foxp3 of CD4^＋CD25^＋Treg cells and suppress the ability of Treg cells to inhibit proliferation of CD4^＋CD25^＋T cells that activated by PHA. Conclusion Bestatin could inhibit the suppressive function of Treg cells,and CD 13 might be a potential molecular target for the immunological interference.