中文摘要：

目的 研究不同管径Ti -TiO2纳米管对成纤维细胞和成骨细胞黏附的影响,探讨最适合细胞黏附的Ti-TiO2纳米管管径.方法 在1、5、10和20V电压下阳极氧化制备Ti-TiO2纳米管组试件,以抛光纯钛作为对照组,场发射扫描电镜观察试件表面形貌；分别接种成纤维细胞和成骨细胞,30、60、120 min后用胞核染色计数方法检测细胞黏附数,扫描电镜观察黏附细胞形态.结果 不同阳极氧化电压可形成15～ 100 nm管径Ti-TiO2纳米管.接种30、60和120 main后,5V纳米管组成纤维细胞黏附数依次为（141±9）、（388±14）和（489±15）个,均比同时间点其余纳米管组少（P ＜0.01）；120 min时20V纳米管组成纤维细胞黏附数为（579±14）个,多于同时间点其余纳米管组（P＜0.01）,但细胞伸展受到抑制.30、60和120 min时5V纳米管组成骨细胞黏附数依次为（198±10）、（431±10）和（501±10）个,除30 min时与对照组差异无统计学意义外,均比同时间点其余组多（P＜0.01）,且细胞伸展良好；3个时间点20V纳米管组成骨细胞黏附数依次为（152±11）、（403 ±9）和（465±12）个,与同时间点其余纳米管组相比黏附数最少（P＜0.05）.结论 纳米管不同程度抑制成纤维细胞的黏附,5V电压制备的纳米管可最大程度促进成骨细胞的黏附,同时抑制成纤维细胞的黏附.

英文摘要：

Objective To study the effect of Ti-TiO2 nanotubes with different diameters on the adhesion of fibroblast and osteoblast,and to find which diameter was more favorable for cells＇ respective adhesion.Methods Pure titanium sheets were polished and then anodized at different potentials for 1 h with Ti as anode and Pt as cathode.TiO2 nanotubes formed at 1,5,10 and 20 V potentials served as experimental groups and polished pure titanium served as control group.Field emission scanning electron microscopy（Fe-SEM） was used to analyze the surface topography.Stained nucleus with Hoechst33342 were used to measure the cell adhesion.The cell shape on the sample surface were analyzed with Fe-SEM.Results TiO2 nanotube array of different inner diameters from 15 nm to 100 nm were grown on titanium sheets by anodization at potentials from 1 to 20 V.At 30,60 and 120 min,fibroblast adhesion at nanotubes anodized at 5 V was （141 ±9）,（388 ± 14） and （489 ± 15）respectively,significantly less than any other nanotube surface at the same time（P 〈0.01）.Nanotubes anodized at 20 V had the least inhibitory effect for fibroblast adhesion with a number of （579 ± 14） at 120 min,and the cell shape was also inhibited.At 30,60 and 120 min,osteoblast had a significant better adhesion on nanotubes formed at 5 V than it did on any other surface at the same time（P 〈0.01）,except the control group at 30 min,with the adhesion number of （198 ± 10）,（431 ± 10） and （501 ± 10） respectively,and osteoblast had a abundant spread on nanotubes formed at 5 V; while osteoblast adhesion on nanotubes anodized at 20 V was （ 152 ± 11 ）,（403 ±9）and （465 ± 12） respectively,less than on any other nanotube surface within the same time（P 〈0.05）,and the cell shape on the surface changed to be more elongate.Conclusions Fibroblast adhesion is inhabited more or less on Ti-TiO2 nanotubes of different diameters.Nanotubes formed at 5 V have the most osteoblast adhesion,and inhibit fibroblast adhesion.