中文摘要：

对RNA干扰（RNAi）表达载体的非生物与生物递送系统及主流应用进行综述，明确其在基因调控及未来基因治疗领域的应用前景，并指出其在种属特异性及表达效率等方面存在的局限性。对RNAi 表达载体的结构组成、发展沿革及体内作用机制进行归纳分析，提出基于结构组成和加工机制双重属性的分类方法。

英文摘要：

This paper reviewed the non-biological and biological delivery systerms of RNA interference expression vector as well as the major applications,and cleared the application prospect in the area of gene regulation and gene therapy in the future,and also pointed out the limitation in the species-specificity and expression efficiency etc.This paper summa-rized and analyzed the structure composition and development evolution of the RNAi expression vector as well as the func-tion mechanism invivo,and proposed the classification based on the dual attributes of structure composition and process-ing mechanism.