中文摘要：

为了建立SYBR GreenⅠ荧光染料实时定量PCR方法,测定TaAGL7-N基因在小麦不同器官的表达水平。采用RT-PCR扩增小麦TaAGL7-N基因片段,建立SYBR GreenⅠ荧光定量PCR标准曲线,以18SrRNA为内参,检测小麦不同器官中的TaAGL7-N表达水平。结果表明,标准曲线循环阈值与模板浓度有良好的线性关系,熔解曲线分别在82.8～83.6℃和83.1～85.6℃各仅有一个单峰。成功建立SYBR GreenⅠ荧光染料实时定量PCR法,该方法具有特异度和敏感度高、稳定性好的特点,可用于定量测定小麦中TaAGL7-N的表达水平。

英文摘要：

The study aimed to establish a real-time PCR with SYBR Green Ⅰ and to detect the expression level of TaAGL7-N gene in different organs of wheat.The target gene TaAGL7-N was amplified with RT-PCR and used as quantitative template to generate standard curve.18SrRNA as the internal standard was performed to detect the 1evels of TaAGL7-N on different growth stages of wheat.The results showed that the standard curve indicated the linear relationship between CT（cycle threshold） and template concentration.The melting cure showed a peak of TaAGL7-N.Peak of 18SrRNA and TaAGL7-N were 82.8-83.6 ℃ and 83.1-85.6 ℃.The method of SYBR Green Ⅰ real-time PCR for TaAGL7-N was sensitive,specific and accurate.It can be used to detect the expression level of TaAGL7-N gene in different organs of wheat.