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中文摘要：
加压毛细管电色谱（pCEC）具有电泳和液相色谱的双重分离机理，其柱效高、选择性强、分辨率高和分离速度快并可进行梯度洗脱。我们在此基础上加入离子交换色谱模式，构建了强阳离子交换一反相加压毛细管液相色谱（micro strongcationex change liquid chromatography／reversed phase pressurized capillary electrochromatogra-phy，μ-SCXLC／RP—pCEC）二维系统，并对中药黄柏的提取物进行了优化分离。第一维μ-SCXLC采用线性盐梯度分离，样品被切割成11个馏分洗脱收集后进入第二维，第二维脱盐后，采用RP—pCEC进行分离分析，梯发洗脱。以中药黄柏提取物为样品，此二维系统的分辨率和峰容量都较一维系统有很大提高，理论峰容量可达900左右，证明构建的二维体系非常适合复杂样品的分离分析。
英文摘要：
Pressurized capillary electrochromatography （pCEC） combines capillary electrophoresis （CE） with high performance liquid chromatography （HPLC）, resulting in high separation efficiency, high selectivity, high resolution and fast speed. In addition, it provides a solvent gradient capability. Based on pCEC, a two-dimensional chromatography was constructed using micro strong cation exchange liquid chromatography and reversed phase pressurized capillary electrochromatography （Iz-SCXLC/RP-pCEC） for the analysis of Cortex Phellodendri extract. A sample was separated by the first dimensional SCX column with a linear salt gradient elution into 11 plugs. Each plug was collected and injected into the second dimensional RP-pCEC column with a linear gradient for further separation. Compared with one-dimensional liquid chromatography, it has higher resolution and larger peak capacity. The two-dimensional chromatographic system is suitable for the separation and analysis of complex samples.
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