以耐碱性燕麦品种Vao-9和碱敏感性品种白燕5号为试验材料,采用盆栽法,用25、50、75和100mmolL^-1碱浓度(Na2CO3和NaHC03按摩尔比1:1混合)进行短期(14d)和长期(28d)胁迫处理,观测两品种根、茎、叶中Na^+、K^+、Ca^2+、Mg^2+吸收及分配特点,并从离子平衡吸收与分配角度,探讨燕麦对碱胁迫的生理适应机制。胁迫处理14d后,燕麦体内Na^+增加,K^+下降,Ca^2+和Mg抖变化不大,且两品种间各器官中4种离子的分配比例差异不显著。胁迫处理28d后,两品种各器官中Na+增幅较大,心、Ca^2+和Mg^2+降幅较大。Vao-9植株体内Na^+、Ca^2+含量大于白燕5号,但0、Mg^2+含量与白燕5号无显著差异,但两品种间各器官中4种离子的分配特点不同;当胁迫浓度达到100mmolL^-1时,与白燕5号相比,Vao-9叶片中少分配5.9个百分点Na^+,多分配13.5个百分点心、28.9个百分点Ca^2+、10.9个百分点Mg^2+,茎中多分配5.4个百分点Na^+,少分配9.8个百分点心,根中少分配28.9个百分点Ca^2+、10.9个百分点Mg^2+,因而Vao-9叶片中Na^+/K^+、Na^+/Ca^2+、Na^+/Mg^2+值较白燕5号低。可见,燕麦通过提高阳离子选择吸收及器官分配能力以适应碱胁迫。
In a pot experiment, an alkali-tolerant oat variety, Vao-9, and an alkali-sensitive variety, Baiyan 5, were exposed to 25, 50, 75, and 100 mmol L-t of alkali stress (molar ratio of Na2CO3:NaHCO3 = 1:1). The contents of Na^+, K^+, Ca^2+, and Mg^2+ ab- sorbed by oat seedling and their distribution characteristics in root, stem, and leaf were measured after a short-term (14 d) or a long-term (28 d) stress treatment. The objective was to understand the physiological adaptation to alkali stress in oat in the view of ion balance absorption and distribution. After short-term stress, the cation contents in oat plants showed the variations of increased Na^+, decreased K^+, and minor changes in Ca^2+ and Mg^2+. Besides, the distribution proportions of the four ions in various organs were not significantly different between the two varieties. Compared to short-term stress, long-term stress resulted in larger in- crease ofNa^+ content and larger decreases of K^+, Ca^2+, and Mg^2+ contents in all organs of both varieties. Vao-9 absorbed more Na^+ and Ca^2+ than Baiyan 5, and the absorptions ofK^+ and Mg^2+ were not significantly different between varieties, but the distribution characteristics of the four ions in various organs were different between the two varieties. Under 100 mmol L-1 alkali stress, 5.9 percent Na^+ in leaf were lower distributed and 13.5 percent K^+, 28.9 Ca^2+, 10.9 Mg^2+ more distributed in Vao-9 than in Baiyan 5, 5.4 percent Na^+ in stem higher and 9.8 K^+ lower in Vao-9 than in Baiyan 5, 28.9 Ca^2+, 10.9 Mg^2+ in root were lower in Vao-9 than in Baiyan 5, As a result, the Na^+ content and the ratios of Na^+/K^+, Na^+/Ca^2+, and Na^+/Mg^2+ in leaf were lower in Vao-9 than in Baiyan 5. Clearly, oat plant has the mechanism of selective absorption and distribution of various cations in different organs in response to alkali stress.