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红外光谱法用于肉苁蓉属中药鉴别研究
  • ISSN号:1000-0593
  • 期刊名称:《光谱学与光谱分析》
  • 时间:0
  • 分类:O657.3[理学—分析化学;理学—化学]
  • 作者机构:[1]中国医学科学院北京协和医学院药用植物研究所濒危药材繁育国家工程实验室,北京100193, [2]清华大学化学系,北京100084, [3]天津中医药大学,天津300193, [4]永宁县本草苁蓉种植基地,宁夏银川750010
  • 相关基金:国家自然科学基金项目(30772727)和国家科技支撑计划项目(2004BA721A36,2006BAI06A13-05)资助
中文摘要:

采用傅里叶变换红外光谱(FTIR)及其二阶导数谱和二维相关红外光谱对传统名贵中药材肉苁蓉、管花肉苁蓉和盐生肉苁蓉进行鉴别研究。结果表明,肉苁蓉与管花肉苁蓉的红外谱图有明显区别,相似系数仅0.6233;而肉苁蓉与盐生肉苁蓉的峰形和峰位均极其相似,相似系数达0.9048,仅从一维图上无法看出两者的成分差异,但通过二阶导数谱中1730(1738),816cm^-1。处的峰形差异可发现二者的一些成分含量存在较明显差异。二维相关谱更直观地反映出三者的差异,它们的自动峰个数均不相同。可见,红外光谱法基本可以实现了对肉苁蓉属3种药材的鉴别,而且具有快速、有效和无损等优点。

英文摘要:

In the present paper, Fourier transform infrared spectroscopy (FTIR) associated with second derivative infrared spectroscopy and two-dimensional correlation spectroscopy (2D-IR) were used to analyze the 3 different sources of Hebra cistanche. The results showed that these IR spectra showed their different macro-fingerprint features: the charactersistic peaks of C. deserticola were located at -1 730, 1 633, 1 156, 1 081, 1 025 and 931 cm^-1 and those of C. tubulosa were at 1 692, 1 631, 1 604, 1 516, 1 265 and 1 023 cm^-1 , respectively. The only two peaks at -1 151 and 1 085 cm 1 of C. salsa were different from that of C. deserticola. As a result, the IR spectrum of C. deserticola could be identified obviously from that of the C. tubulosa, whose similar index was only 0. 623 3, but it was very similar to that of C. salsa, whose similar index was up to 0. 904 8, demonstrating very similar ingredients. However, the difference between C. deserticola and C. salsa was obvious in the second derivative IR spectra: the shape of the two peaks of C. deserticola at -1 730 and 816 cm^-1 were much sharper. In addition, the fingerprint characters in 2D-IR spectra were more visualized. The three kinds of herbs were quite different from each other in the number and intensity of autopeaks. Therefore, FTIR macro-fingerprint method can identify different sources of Hebra cistance fast, nondestructively and effectively.

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期刊信息
  • 《光谱学与光谱分析》
  • 中国科技核心期刊
  • 主管单位:中国科学技术协会
  • 主办单位:中国光学学会
  • 主编:高松
  • 地址:北京海淀区魏公村学院南路76号
  • 邮编:100081
  • 邮箱:chngpxygpfx@vip.sina.com
  • 电话:010-62181070
  • 国际标准刊号:ISSN:1000-0593
  • 国内统一刊号:ISSN:11-2200/O4
  • 邮发代号:82-68
  • 获奖情况:
  • 1992年北京出版局编辑质量奖,1996年中国科协优秀科技期刊奖,1997-2000获中国科协择优支持基础性高科技学术期刊奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),荷兰文摘与引文数据库,美国工程索引,美国生物医学检索系统,美国科学引文索引(扩展库),英国科学文摘数据库,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国英国皇家化学学会文摘,中国北大核心期刊(2000版)
  • 被引量:40642