中文摘要：

目的研究腺苷（AD）对下丘脑视前正中核（MnPN）神经元调控大鼠睡眠-觉醒周期的影响。方法将SD大鼠随机分为3组：人工脑脊液（ACSF）组、AD组和硝基苯硫嘌呤核苷（NBTI）组,每组5只。采用脑立体定位、微透析和多导睡眠描计技术观察MnPN区给予AD、NBTI对大鼠睡眠-觉醒周期的影响。结果 MnPN区微透析AD后,大鼠非快速眼动（NREM）睡眠增加19.4%（P〈0.01）、觉醒（W）减少26.1%（P〈0.01）、快速眼动（REM）睡眠无明显改变,总睡眠时间（TST）增加17.7%（P〈0.01）。MnPN区微透析NBTI后,大鼠NREM增加11.6%（P〈0.05）,W减少16.7%（P〈0.05）,REM睡眠无明显改变,TST增加11.0%（P〈0.05）。结论 AD在MnPN区参与睡眠-觉醒周期的调节并具有促睡眠作用。

英文摘要：

Objective To investigate the effect of adenosine（AD）in median preoptic mucleus（MnPN）on sleep-wakefulness cycle of rat.Methods Rats were randomly divided into three groups,control group（artificial cerebrospinal fluid group,ACSF）,AD group,nitrobenzylthioinosine（NBTI）group.The effects of AD and NBTI,a sensitive AD transporter,on sleep-wakefulness cycle were studied with the methods of brain stereotaxic,microdialysis,polysomngraphy.Results After microdialyzed AD into MnPN of rat,compared with the control group,the ratio of non-rapid eye movement（NREM） sleep was enhanced by 19.4%（P〈0.01）,wake（W） was decreased by 26.1%（P〈0.01）,total sleep time（TST） was enhanced by 17.7%（P〈0.01）,and REM sleep was shown no significant change.After microdialyzed NBTI into MnPN of rat,compared with the control group,the ratio of NREM sleep was enhanced by 11.6%（P〈0.05）,W was decreased by 16.7%（P〈0.05）,TST was increased by 11.0%（P〈0.05）,and it had no effect on REM sleep.Conclusion AD in MnPN takes part in sleep-wakefulness cycle regulation and facilitates sleep.