中文摘要：

目的 制备急性小鼠海马脑片,观察并分析海马脑片CA1和CA3神经元的电生理特性.方法 将急性制备的小鼠海马脑片,应用红外微分干涉相差技术结合电荷耦合成像系统和脑片膜片钳技术,对小鼠海马CA1和CA3神经元结构特点和电生理特性进行观察和检测.结果 海马CA1和CA3神经元立体结构清晰,胞体突起明显,细胞死亡少且容易封接.海马CA1和CA3神经元自发放电电流和动作电位的幅度、频率,静息膜电位和峰峰间距,二者之间差异均无统计学意义;去极化脉冲电压与钠和钾离子通道电流密度,步阶脉冲电流与动作电位发放数二者之间差异均有统计学意义（P＜0.05）.结论 海马CA1和CA3神经元的一些基本电生理特性存在异同,这结果为记忆和神经疾病等研究提供一定的理论基础.

英文摘要：

Objective Acute mouse hippocampal slices were prepared for patch clamp recording in order to observe the basic electrophysiological properties of hippocampal CA1 and CA3 neurons.Methods The structure properties and basic electrophysiological properties of mouse hippocampal slices were observed and recorded by infrared differential interference contrast technique and patch clamp,respectively.Results The hippocampal slice structure was observed clearly with healthy gloss and few dead neurons,and the hippocampal neurons were sealed easily with patch clamp technique.The spontaneous discharge current and action potential between CA1 and CA3 neurons did not show significant differences in amplitude,frequency,inter spike-intervals and resting membrane potentials.The current density of sodium and potassium ion when injecting pulse stimulations and the action potential numbers when injecting step currents showed significant differences in CA1 and CA3 neurons （P ＜ 0.05）.Conclusion Some basic electrophysiological properties of hippocampal CA1 and CA3 neurons provide certain theoretical basis for memory and nerve diseases research.