中文摘要：

背景：前期研究已证实，烟酰胺能够促进椎间盘细胞的增殖并对白细胞介素1B致椎间盘退变起到保护作用，而烟酰胺对椎间盘退变的保护机制还不很清楚。目的：观察烟酰胺对白细胞介素1β诱导体外培养的兔椎间盘细胞凋亡及能量代谢相关基因表达的影响。设计、时间及地点：实验于2007-11／2008—05在华中科技大学同济医学院附属协和医院中心实验室完成。材料：3～4月龄日本大白兔10只，体质量1．5～2．Okg，取LM脊柱节段56个椎间盘组织，用于构建兔椎间盘组织凝胶培养模型。方法：将56枚椎间盘随机分为4组，每组14枚。正常对照组：不加入药物：烟酰胺组：加入0．5g／L烟酰胺；退变组：加入10μg/L白细胞介素1β;治疗组加入10μg／L白细胞介素1β及0．5g／L烟酰胺。培养2周后对各组标本行TUNEL染色及Fas，Caspase-3，Bcl-2，低氧诱导因子1d、葡萄糖转运体1、血管内皮细胞生长因子免疫组织化学染色。主要观察指标：各组标本TUNEL染色及免疫组织化学染色阳性细胞率。结果：TUNEL染色示加入烟酰胺后退变组阳性细胞率较正常对照组上升（P=0．001）。Fas染色退变组阳性细胞率较正常对照组明显上升（P〈0．01）。Bcl-2染色示烟酰胺组较正常对照组阳性细胞率升高（P=0．004）。Caspase-3染色示治疗组阳性细胞率较退变组下降（P=0．024），但仍高于正常对照组（P=0.006）。低氧诱导因子1d染色示烟酰胺组阳性细胞率低于正常对照组（P〈0．01）；退变组阳性细胞率较正常对照组上升（P〈0．01）。葡萄糖转运体1染色示治疗组阳性细胞率高于正常对照组（P〈0．01）。结论：烟酰胺可以抑制白细胞介素1β诱导的椎间盘细胞凋亡，改善白细胞介素10导致的椎间盘能量代谢障碍。

英文摘要：

BACKGROUND： Studies have reported that Niacinamide is capable of promoting the proliferation of intervertebral cell and protecting intervertebral disc （IVD） against interleukin-1β -induced degeneration. However, the mechanism of Niacinamide underlying protecting IVD degeneration remains uncertain. OBJECTIVE： To investigate the regulatory effect of Niacinamide on interleukin-1β -induced cell apoptosis and energy metabolism related gene in IVD in vitro. DESIGN, TIME AND SETTING： Experiments were performed in the Central Laboratory of Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology from November 2007 to May 2008. MATERIALS： Fifty-six IVDs from L1 6 lumbar spine of ten Japanese white rabbits, aged 3-4 months and weighing 1.5 2.0 kg, were.harvested and cultured in alginate gel for further experiments. METHODS： IVD cultured models were randomly divided into 4 groups： normal control group （with the absence of drugs）, Niacinamide group （administrating 0.5 g/L Niacinamide）, degeneration group （administrating 10 μg/L interleukin-1β ）, treatment group （administrating 10 v g/L interleukin-1β and 0.5 g/L Niacinamide）. After 2 weeks of culture, TUNEL staining and immunohistochemical staining for FAS, Bcl-2, Caspase-3, hypoxia induced factor 1 o, glucose transporter-1 and vascular endothelial cell growth factor were used to detect alternated cell apoptosis and expression of energy metabolism related genes. MAIN OUTCOME MEASURES： The positive cell rates of TUNEL staining and immunohistochemical staining in each group. RESULTS： The rate of TUNEL positive-staining cells of degeneration group was higher than normal control group （P=0.001）. The rate of FAS positive-staining cells of degeneration group was obviously higher than normal control group （P 〈 0.01）. The rate of Bcl-2 positive-staining cells of Niacinamide group was higher than normal control group （P=0.004）. The rate of Caspase-3 positive-staining cells of treatment gr