中文摘要：

目的：探究纤维素酶酶法制备稠李花色苷的最佳条件,并研究制备的稠李花色苷对H2O2诱导的大鼠胰岛素瘤（Ins-1）细胞损伤的保护作用。方法：通过单因素试验和正交试验,优化得到酶法制备稠李花色苷的最佳条件;稠李花色苷处理大鼠Ins-1细胞,进行形态学观察、3-（4,5-二甲基噻唑-2）-2,5-二苯基四氮唑溴盐[3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide,MTT]细胞活力实验、2’,7’-二氢二氯荧光素二乙酸酯（2’,7’-dichloro dihydrofluorescein diacetate,DCFH-DA）荧光探针法检测细胞内活性氧实验研究稠李花色苷对H2O2诱导损伤的Ins-1细胞保护作用。结果：纤维素酶法提取稠李花色苷的最佳条件为：温度60℃、纤维素酶添加量9 mg/g、料液比1∶35（g/m L）、p H 3.5,此条件下稠李花色苷得率为（0.956±0.027）mg/g;形态学观察及MTT细胞活力实验显示,稠李花色苷对H2O2诱导损伤的Ins-1细胞具有较强的保护作用,DCFH-DA法检测细胞内活性氧实验说明,稠李花色苷能够显著地清除Ins-1细胞内的活性氧。结论：纤维素酶法制备稠李花色苷是一种有效的方法,制备的稠李花色苷对H2O2诱导的大鼠Ins-1细胞损伤具有较强的保护作用。

英文摘要：

Purpose： To optimize the extraction conditions for anthocyanins from Padus racemosa fruits by cellulose hydrolysis, and to detect the protective effect of the extracted anthocyanins on H2O2-induced damage in Ins-1 cells. Methods： The optimization was performed by single factor and orthogonal array experiments. The protective effect of Padus racemosaanthocyanins on Ins-1 cells were examined by morphological observation, MTT assay and DCFH-DA fluorescent probe method. Results： The optimal extraction conditions were achieved by carrying out cellulose hydrolysis at 60 ℃, p H 3.5 and an enzyme dosage of 9 mg/g with a solid/liquid ratio of 1：35（g/m L）. Under these conditions, the yield of Padus racemosaanthocyanins was（0.956 ± 0.027） mg/g. Padus racemosa anthocyanins protected Ins-1 cells from damage induced by H2O2 as indicated by morphological observation and MTT assay, and strongly scavenged reactive oxygen species in the DCFHDA fluorescent assay. Conclusions： Enzymatic hydrolysis is an effective method for extracting anthocyanins from Padus racemosa, and the extracted anthocyanins strongly protect against H2O2-induced damage in Ins-1 cells.