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中文摘要：
以鸡腿菇菌丝体为原料,采用超声波联合酶法提取鸡腿菇菌丝体多糖,通过单因素试验和L9（34）正交试验考察超声功率、纤维素酶用量、提取温度、提取时间对鸡腿菇菌丝体多糖提取的影响,确定多糖最佳提取条件。利用Sephadex G-200凝胶过滤和DEAE-32离子交换层析纯化鸡腿菇菌丝体多糖,紫外扫描和薄膜电泳方法鉴定多糖的纯度。结果表明：鸡腿菇菌丝体多糖最佳提取条件为超声功率500W、纤维素酶加酶量900U/g（以菌丝体干质量计）、提取时间2.5h、提取温度55℃,最适条件下菌丝体的多糖提取量为（3.48±0.17）%。纯化得到的两种主要多糖薄膜电泳显示均为单一条带,证明纯化的多糖达到了电泳纯。
英文摘要：
Crude polysaccharides were obtained from Coprinus comatus mycelia using ultrasonication followed by cellulase hydrolysis.The optimum extraction conditions that provided maximum extraction efficiency（3.48 ± 0.17） % were 500 W,900 U/g（relative to the dry weight of Coprinuscomatus mycelia）,2.5 h and 55 ℃ for microwave power,enzyme dose,extraction time and temperature,respectively as determined by orthogonal array design.The crude polysaccharides were purified by Sephadex G-200 and DEAE-32 column chromatography to obtain two major fractions.Both frations displayed a single homogeneous band in cellulose acetate electrophoresis.Thus electrophoresis-pure polysaccharides were obtained.
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