中文摘要：

目的：采用双向电泳和免疫印迹技术对软叶针葵花粉提取液蛋白质组分进行分析,寻找软叶针葵花粉的主要变应原。方法：提取软叶针葵花粉变应原,应用双向电泳（2-DE）结合免疫印迹技术,获得相应的电泳图谱和免疫印迹图谱。结果：软叶针葵花粉蛋白提取液经双向电泳后,用考马斯亮蓝染色,得到的电泳图显示约有601个主要蛋白斑点。分子量（Mr）分布约20～130kD;等电点（pI）主要集中在4.0～8.0。免疫印迹分析结果显示,阳性组与对照组的差异蛋白点数约为19个。结论：双向电泳联合免疫印迹技术对软叶针葵花粉提取液蛋白质组分分析,可寻找到其主要变应原,并可进一步用于变应原致敏组分的研究。

英文摘要：

Objective：To investigate allergen extract and to seek the main allergens of phoenix roebelenii pollen by the technology of two dimensional electrophoresis（2-DE）in protein analysis and immunoblotting.Method：Phoenix roebelenii pollen allergen extract was prepared with routine method,2-DE combined with immunoblotting assay（Western Blotting）,laser imagescanning,2-DE gel analysis software were used to analyzing its protein components.Result：About 601 soluble proteins spots were revealed in coomassie-stained gels.Most of the proteins had a molecular weight（Mr）of 20 to 130kD,and an isoelectric point（pI）value of 4.0to 8.0.Immunoblotting of 2-DE were showed and there were 19 specific antigen spots,compared with the controls.Conclusion：2-DE is a good method in protein analysis of ephemeroptera allergen extract,and it is useful in seeking main allergens and further research for allergic components.